Single Holliday junctions are intermediates of meiotic recombination

被引:160
作者
Cromie, Gareth A.
Hyppa, Randy W.
Taylor, Andrew F.
Zakharyevich, Kseniya
Hunter, Neil
Smith, Gerald R.
机构
[1] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA
[2] Univ Calif Davis, Microbiol Sect, Davis, CA 95616 USA
[3] Univ Calif Davis, Sect Mol & Cellular Biol, Davis, CA 95616 USA
关键词
D O I
10.1016/j.cell.2006.09.050
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Crossing-over between homologous chromosomes facilitates their accurate segregation at the first division of meiosis. Current models for crossing-over invoke an intermediate in which homologs are connected by two crossed-strand structures called Holliday junctions. Such double Holliday junctions are a prominent intermediate in Saccharomyces cerevisiae meiosis, where they form preferentially between homologs rather than between sister chromatids. In sharp contrast, we find that single Holliday junctions are the predominant intermediate in Schizosaccharomyces pombe meiosis. Furthermore, these single Holliday junctions arise preferentially between sister chromatids rather than between homologs. We show that Mus81 is required for Holliday junction resolution, providing further in vivo evidence that the structure-specific endonuclease Mus81-Eme1 is a Holliday junction resolvase. To reconcile these observations, we present a unifying recombination model applicable for both meiosis and mitosis in which single Holliday junctions arise from single- or double-strand breaks, lesions postulated by previous models to initiate recombination.
引用
收藏
页码:1167 / 1178
页数:12
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