Near-ultraviolet photolysis of L-mandelate, formation of reactive oxygen species, inactivation of phage T7 and implications on human health

被引:3
作者
Ahmad, SI
Hargreaves, A
Taiwo, FA
Kirk, SH
机构
[1] Nottingham Trent Univ, Sch Biomed & Nat Sci, Nottingham NG11 8NS, England
[2] De Montfort Univ, Sch Pharm, Leicester LE1 9BH, Leics, England
关键词
near ultraviolet light; (L)-mandelate; reactive oxygen species; electron transfer reaction; phage T7;
D O I
10.1016/j.jphotobiol.2004.08.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
Compared with ultraviolet B and C, UVA is considered to have little direct effects on biological systems. However, damaging effects of UVA on biological systems are often synergistically enhanced in the presence of sensitizers. Production of reactive oxygen species (ROS) has been implicated in the process. Several ROS have been identified but their involvement in inducing cellular damage is yet to be fully evaluated. Although membranes and proteins are affected, DNA is an important target and a variety of types of damage have been reported. Here, we present evidence that L-mandelate can act as a near UV (NLTV) sensitizer, when activated by a lamp emitting 99% UVA and 1% UVB. Although evidence is available that H2O2 and a small amount of (OH)-O-. are produced, an alternative effect of the sensitization reaction may involve direct electron transfer. Studies have shown that NUV photolysis of mandelate can inactivate phage T7. Employment of tetrazolium blue test to detect superoxide anion may not be sufficient evidence as this agent may be reduced by alternative routes. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:55 / 62
页数:8
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