Arrays of mobile tethered vesicles on supported lipid bilayers

被引：200

作者：

Yoshina-Ishii, C ^{[1
]}

Boxer, SG ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 13期

关键词：

D O I：

10.1021/ja029783+

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a new system of laterally mobile, arrayed vesicles that are encoded with DNA to control tethering to fluid-supported phospholipid bilayers. The motion of individual fluorescently labeled vesicles, specifically bound, are easily visualized by fluorescence video microscopy and observed to collide reversibly on the surface. This system is an ideal model for studying interactions involving membranes, in particular integral membrane proteins. Copyright © 2003 American Chemical Society.

引用

收藏

页码：3696 / 3697

页数：2

相关论文

共 17 条

[1] Immobilization in surface-tethered lipid vesicles as a new tool for single biomolecule spectroscopy [J].

Boukobza, E ;

Sonnenfeld, A ;

Haran, G .

JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (48) :12165-12170

[2] Electrical manipulation of glycan phosphatidyl inositol tethered proteins in planar supported bilayers [J].

Groves, JT ;

Wulfing, C ;

Boxer, SG .

BIOPHYSICAL JOURNAL, 1996, 71 (05) :2716-2723

[3] Electric field-induced concentration gradients in planar supported bilayers [J].

Groves, JT ;

Boxer, SG .

BIOPHYSICAL JOURNAL, 1995, 69 (05) :1972-1975

[4] Micropattern formation in supported lipid membranes [J].

Groves, JT ;

Boxer, SG .

ACCOUNTS OF CHEMICAL RESEARCH, 2002, 35 (03) :149-157

[5] DNA microarray technology: Devices, systems, and applications [J].

Heller, MJ .

ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2002, 4 :129-153

[6] Patterning barriers to lateral diffusion in supported lipid bilayer membranes by blotting and stamping [J].

Hovis, JS ;

Boxer, SG .

LANGMUIR, 2000, 16 (03) :894-897

[7] Quantification of tight binding to surface-immobilized phospholipid vesicles using surface plasmon resonance:: Binding constant of phospholipase A2 [J].

Jung, LS ;

Shumaker-Parry, JS ;

Campbell, CT ;

Yee, SS ;

Gelb, MH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (17) :4177-4184

[8] Formation of supported lipid bilayer composition arrays by controlled mixing and surface capture [J].

Kam, L ;

Boxer, SG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (51) :12901-12902

[9] Patterning hybrid surfaces of proteins and supported lipid bilayers [J].

Kung, LA ;

Kam, L ;

Hovis, JS ;

Boxer, SG .

LANGMUIR, 2000, 16 (17) :6773-6776

[10] DIRECT OBSERVATION OF BROWNIAN-MOTION OF LIPIDS IN A MEMBRANE [J].

LEE, GM ;

ISHIHARA, A ;

JACOBSON, KA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (14) :6274-6278