UK114, a YjgF/Yer057p/UK114 family protein highly conserved from bacteria to mammals, is localized in rat liver peroxisomes

被引：8

作者：

Antonenkov, Vasily D.

Ohlmeier, Steffen

Sormunen, Raija T.

Hiltunen, J. Kalervo

机构：

[1] Oulu Univ, Bioctr, Dept Biochem, FIN-90014 Oulu, Finland

[2] Oulu Univ, Bioctr, Prote Core Facil, FIN-90014 Oulu, Finland

[3] Oulu Univ, Bioctr, Dept Pathol, FIN-90014 Oulu, Finland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 357卷 / 01期

基金：

芬兰科学院;

关键词：

Peroxisomes; UK114; YjgF/Yer057p/UK114 protein family; clofibrate; rat liver;

D O I：

10.1016/j.bbrc.2007.03.136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mammalian UK114 belongs to a highly conserved family of proteins with unknown functions. Although it is believed that UK114 is a cytosolic or mitochondrial protein there is no detailed study of its intracellular localization. Using analytical subcellular fractionation, electron microscopic colloidal gold technique, and two-dimensional gel electrophoresis of peroxisomal matrix proteins combined with mass spectrometric analysis we show here that a large portion of UK114 is present in rat liver peroxisomes. The peroxisomal UK114 is a soluble matrix protein and it is not inducible by the peroxisomal proliferator clofibrate. The data predict involvement of UK114 in peroxisomal metabolism. (c) 2007 Elsevier Inc. All rights reserved.

引用

收藏

页码：252 / 257

页数：6

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