Glycosylated Quantum Dots for the Selective Labelling of Kluyveromyces bulgaricus and Saccharomyces cerevisiae Yeast Strains

被引：21

作者：

Coulon, Joel ^{[1
]}

Thouvenin, Ilan ^{[1
]}

Aldeek, Fadi ^{[1
]}

Balan, Lavinia ^{[3
]}

Schneider, Raphael ^{[2
]}

机构：

[1] Nancy Univ, LCPME, CNRS, F-54600 Villers Les Nancy, France

[2] Nancy Univ, DCPR, CNRS, F-54001 Nancy, France

[3] Univ Haute Alsace, CNRS, DPG, ENSCMu,UMR 7525, F-68093 Mulhouse, France

来源：

JOURNAL OF FLUORESCENCE | 2010年 / 20卷 / 02期

关键词：

Quantum dots; Cadmium telluride; Yeast; Labelling; Lectins/saccharides selective recognition; CDTE NANOCRYSTALS; FLOCCULATION; SURFACE; LECTIN; CELLS; PROTEIN; PROBES;

D O I：

10.1007/s10895-009-0590-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Highly fluorescent CdTe quantum dots (QDs) stabilized by thioglycolic acid (TGA) were prepared by an aqueous solution approach and used as fluorescent labels in detecting yeast cells. Sugars (mannose, galactose or glucose) were adsorbed on CdTe@TGA QDs and the interaction of these nanoparticles with yeast cells was studied by fluorescence microscopy. Results obtained demonstrate that galactose and mannose functionalized QDs associate respectively with Kluyveromyces bulgaricus and Saccharomyces cerevisiae yeast strains due to saccharide/lectin specific recognition. Glucose-functionalized CdTe QDs, which are not recognized by cell lectins, preferentially localize in the bud scars of S. cerevisiae.

引用

页码：591 / 597

页数：7

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