Nitric oxide Plays a Role as Second Messenger in the Ultraviolet-B Irradiated Green Alga Chlorella pyrenoidosa

被引：14

作者：

Chen, K. ^{[1
]}

Song, L. ^{[2
]}

Rao, B. ^{[1
]}

Zhu, T. ^{[1
]}

Zhang, Y. T. ^{[1
]}

机构：

[1] Xinyang Normal Univ, Dept Biol, Xinyang 46400, Henan Province, Peoples R China

[2] Xinyang Normal Univ, Dept Chem, Xinyang 46400, Henan Province, Peoples R China

来源：

FOLIA MICROBIOLOGICA | 2010年 / 55卷 / 01期

关键词：

MEMBRANE H+-ATPASE; OXIDATIVE STRESS; BLUE-GREEN; HYPOCOTYL ELONGATION; NITRATE REDUCTASE; WATER-STRESS; UV EXPOSURE; DAMAGE; 5-NUCLEOTIDASE; SURVIVABILITY;

D O I：

10.1007/s12223-010-0009-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nitric oxide (NO) stimulated the activity of plasma membrane H+-ATPase, 5'-nucleotidase, peroxidase, ascorbate peroxidase and glutathione reductase in ultraviolet B (UV-B) irradiated Chlorella pyrenoidosa. It also boosted the activity of nitrogen-metabolism enzymes such as nitrate reductase, nitrite reductase, glutamine synthetase, which were inhibited by UV-B irradiation. The chlorophyll fluorescence ratio (Fv/Fm) of the UV-B irradiated algae and decreased continuously after the cells were transferred to UV-B irradiation. A continuing decrease of the Fv/Fm was observed even after the cells were transferred to photosynthetically active radiation (PAR). After adaptation for 8 h under PAR (after treatment with nitric oxide), Fv/Fm recovered to 55% of normal levels - without NO the value approached zero. Exogenous NO stopped the decay of chlorophyll and thylakoid membrane in cells exposed to UV-B irradiation. NO plays probably a key role in damage induced by UV-B irradiation in green algae.

引用

页码：53 / 60

页数：8

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