In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease

被引：45

作者：

Cordova, I

Jones, P ^{[1
]}

Harrison, NA

Oropeza, C

机构：

[1] Rothamsted Res, Plant Pathogen Interact Div, Harpenden AL5 2JQ, Herts, England

[2] Univ Florida, Ft Lauderdale Res & Educ Ctr, Dept Plant Pathol, Ft Lauderdale, FL 33314 USA

[3] Ctr Invest Cientif Yucatan, Merida, Mexico

来源：

MOLECULAR PLANT PATHOLOGY | 2003年 / 4卷 / 02期

关键词：

D O I：

10.1046/j.1364-3703.2003.00152.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

DNA of the lethal yellowing (LY) phytoplasma was detected in 13 of 72 embryos from fruits of four diseased Atlantic tall coconut palms by polymerase chain reaction (PCR) assays employing phytoplasma universal rRNA primer pair P1/P7, nested LY group-specific rRNA primer pair 503f/LY16Sr or LY phytoplasma-specific nonribosomal primer pair. LYF1/R1. Phytoplasma distribution in sectioned tissues from six PCR positive embryos was determined by in situ PCR and digoxigenin-11-deoxy-UTP (Dig) labelling of amplification products. Dig-labeled DNA products detected by colourimetric assay were clearly evident on sections from the same three embryos investigated in detail by in situ PCRs employing primer pairs P1/P7 or LYF1/R1. Deposition of blue-green stain on sections as a result of each assay was restricted to areas of the embryos corresponding to the plumule and cells ensheathing it. By comparison, similarly treated embryo sections derived from fruits of a symptomless Atlantic tall coconut palm were consistently devoid of any stain. Presence of phytoplasma DNA in embryo tissues suggests the possible potential for seed transmission which remains to be demonstrated.

引用

页码：99 / 108

页数：10

共 48 条

[1] Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants [J].