High-efficiency gene delivery for expression in mammalian cells by nanoprecipitates of Ca-Mg phosphate

被引：66

作者：

Chowdhury, EH ^{[1
]}

Kunou, M ^{[1
]}

Nagaoka, M ^{[1
]}

Kundu, AK ^{[1
]}

Hoshiba, T ^{[1
]}

Akaike, T ^{[1
]}

机构：

[1] Tokyo Inst Technol, Dept Biomol Engn, Grad Sch Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

GENE | 2004年 / 341卷

基金：

日本学术振兴会;

关键词：

transfection; DNA uptake; crystal growth; nanoparticles;

D O I：

10.1016/j.gene.2004.07.015

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

Transfer of desirable genetic sequences into mammalian cells is an essential tool for analysis of gene structure, functions and regulation and industry-based production of therapeutically important proteins and pivotal for gene therapy and DNA vaccination strategies. Considering some severe limitations of viral systems including immunogenicity, carcinogenicity and so on, synthetic nonviral systems are highly desirable in the above applications. However, existing nonviral techniques are extremely inefficient compared to the viral ones. Therefore, we report here on the development of a highly efficient synthetic device for gene delivery and expression into mammalian cells, based on controllable growth of nanoapatite particles. Mg2+ incorporation into the apatite particles caused significant inhibition of particle growth, resulting in retention of nanosized particles which contributed remarkably to the cellular uptake of DNA and its subsequent expression (>10-fold) compared with classical calcium phosphate coprecipitation, one of the most widely used transfection methods. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：77 / 82

页数：6

共 23 条

[1]

In vivo gene transfer to dopamine neurons of rat substantia nigra via the high-affinity neurotensin receptor [J].