Assessment of strain field in endothelial cells subjected to uniaxial deformation of their substrate

被引:80
作者
Caille, N [1 ]
Tardy, Y [1 ]
Meister, JJ [1 ]
机构
[1] Swiss Federal Inst Technol, PSE Ecublens, Biomed Engn Lab, CH-1015 Lausanne, Switzerland
关键词
cellular mechanics; strain field; nucleus deformation; stretch chamber; confocal microscopy; fluorescent beads;
D O I
10.1114/1.132
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
A stretch chamber has been developed in order to visualize the deformation of cells subjected to controlled uniaxial stretch of their substrate. A rectangular, custom-made, transparent silicone channel is used as a deformable substrate. Bovine aortic endothelial cells are plated at the bottom of the channel whose lateral deformation is controlled by two piezoelectric translators. The system is mounted on the stage of a confocal microscope where three-dimensional (3D) images of the cells can be acquired simultaneously in the three RGB channels. The first channel provides images of 216 nm fluorescent beads embedded in the cytoskeleton (used as internal markers). The second is used to image the shape of the nucleus revealed by live cell nucleic acid staining. The third one pro vides a transmitted light image of the cell outline. 3D images of the cell are taken before deformation, after uniaxial deformation of the substrate (up to 25%) and after relaxation. Results indicate that: (a) the cell closely follows the deformation imposed by the substrate with no measurable residual strain after relaxation, and (b) there is a clear mechanical coupling between the extracellular matrix and the nucleus, which deforms significantly under the applied substrate stretch. Suggesting that the nucleus can directly sense the mechanical environment of the cell, the latter result has potentially important implications for signal transduction. (C) 1998 Biomedical Engineering Society.
引用
收藏
页码:409 / 416
页数:8
相关论文
共 19 条
[1]   FORCE GENERATION OF ORGANELLE TRANSPORT MEASURED INVIVO BY AN INFRARED-LASER TRAP [J].
ASHKIN, A ;
SCHUTZE, K ;
DZIEDZIC, JM ;
EUTENEUER, U ;
SCHLIWA, M .
NATURE, 1990, 348 (6299) :346-348
[2]   STRAIN-MEASUREMENTS IN CULTURED VASCULAR SMOOTH-MUSCLE CELLS SUBJECTED TO MECHANICAL DEFORMATION [J].
BARBEE, KA ;
MACARAK, EJ ;
THIBAULT, LE .
ANNALS OF BIOMEDICAL ENGINEERING, 1994, 22 (01) :14-22
[3]   MECHANICAL-STRESS MECHANISMS AND THE CELL - AN ENDOTHELIAL PARADIGM [J].
DAVIES, PF ;
TRIPATHI, SC .
CIRCULATION RESEARCH, 1993, 72 (02) :239-245
[4]   THE DYNAMIC-RESPONSE OF VASCULAR ENDOTHELIAL-CELLS TO FLUID SHEAR-STRESS [J].
DEWEY, CF ;
BUSSOLARI, SR ;
GIMBRONE, MA ;
DAVIES, PF .
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 1981, 103 (03) :177-185
[5]   SENSITIVE FORCE TECHNIQUE TO PROBE MOLECULAR ADHESION AND STRUCTURAL LINKAGES AT BIOLOGICAL INTERFACES [J].
EVANS, E ;
RITCHIE, K ;
MERKEL, R .
BIOPHYSICAL JOURNAL, 1995, 68 (06) :2580-2587
[6]   EPITHELIAL CYTOSKELETAL FRAMEWORK AND NUCLEAR MATRIX INTERMEDIATE FILAMENT SCAFFOLD - 3-DIMENSIONAL ORGANIZATION AND PROTEIN-COMPOSITION [J].
FEY, EG ;
WAN, KM ;
PENMAN, S .
JOURNAL OF CELL BIOLOGY, 1984, 98 (06) :1973-1984
[7]   ENDOTHELIAL NUCLEAR PATTERNS IN CANINE ARTERIAL TREE WITH PARTICULAR REFERENCE TO HEMODYNAMIC EVENTS [J].
FLAHERTY, JT ;
FERRANS, VJ ;
TUCKER, WK ;
FRY, DL ;
PATEL, DJ ;
PIERCE, JE .
CIRCULATION RESEARCH, 1972, 30 (01) :23-&
[9]  
IVES CL, 1986, IN VITRO CELL DEV B, V22, P500
[10]  
MCINTOSH FC, 1995, PHYS REV LETT, V75, P4425