Tir tyrosine phosphorylation and pedestal formation are delayed in enteropathogenic Escherichia coli sepZ::TnphoA mutant 30-5-1(3)

被引：12

作者：

DeVinney, R

Nisan, I

Ruschkowski, S

Rosenshine, I

Finlay, BB ^{[1
]}

机构：

[1] Univ British Columbia, Biotechnol Lab, Vancouver, BC V6T 1Z3, Canada

[2] Hebrew Univ Jerusalem, Dept Mol Genet, IL-91120 Jerusalem, Israel

[3] Hebrew Univ Jerusalem, Dept Biotechnol, IL-91120 Jerusalem, Israel

[4] Hebrew Univ Jerusalem, Dept Clin Microbiol, IL-91120 Jerusalem, Israel

来源：

INFECTION AND IMMUNITY | 2001年 / 69卷 / 01期

关键词：

D O I：

10.1128/IAI.69.1.559-563.2001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Enteropathogenic Escherichia call (EPEC) strain 30-5-1(3) has been reported to form attaching and effacing (A/E) lesions without Tir tyrosine phosphorylation. In this study, we show that 30-5-1(3), which has a transposon insertion within the sepZ gene, forms wild-type A/E lesions including Tir tyrosine phosphorylation, but at a slower rate. A/E lesion formation by 30-5-1(3) occurs without detectable secretion of Tir or other EPEC Esp secreted proteins.

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页码：559 / 563

页数：5

相关论文

共 24 条

[1] Enteropathogenic Escherichia coli O103 from rabbit elicits actin stress fibers and focal adhesions in HeLa epithelial cells, cytopathic effects that are linked to an analog of the locus of enterocyte effacement [J].

DeRycke, J ;

Comtet, E ;

Chalareng, C ;

Boury, M ;

Tasca, C ;

Milon, A .

INFECTION AND IMMUNITY, 1997, 65 (07) :2555-2563

[2] Initial binding of Shiga toxin-producing Escherichia coli to host cells and subsequent induction of actin rearrangements depend on filamentous EspA-containing surface appendages [J].

Ebel, F ;

Podzadel, T ;

Rohde, M ;

Kresse, AU ;

Krämer, S ;

Deibel, C ;

Guzmán, CA ;

Chakraborty, T .

MOLECULAR MICROBIOLOGY, 1998, 30 (01) :147-161

[3] The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69 [J].

Elliott, SJ ;

Wainwright, LA ;

McDaniel, TK ;

Jarvis, KG ;

Deng, YK ;

Lai, LC ;

McNamara, BP ;

Donnenberg, MS ;

Kaper, JB .

MOLECULAR MICROBIOLOGY, 1998, 28 (01) :1-4

[4] BipA affects Ca++ fluxes and phosphorylation of the translocated intimin receptor (Tir/Hp90) in host epithelial cells infected with enteropathogenic E-coli [J].

Farris, M ;

Grant, A ;

Jane, S ;

Chad, J ;

O'Connor, CD .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1998, 26 (03) :S225-S225

[5] Hierarchy in the expression of the locus of enterocyte effacement genes of enteropathogenic Escherichia coli [J].

Friedberg, D ;

Umanski, T ;

Fang, YA ;

Rosenshine, I .

MOLECULAR MICROBIOLOGY, 1999, 34 (05) :941-952

[6]

GOMEZDUARTE OG, 1995, INFECT IMMUN, V63, P1767

[7] Type III protein secretion systems in bacterial pathogens of animals and plants [J].

Hueck, CJ .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (02) :379-+

[8] Divergent signal transduction responses to infection with attaching and effacing Escherichia coli [J].

Ismaili, A ;

McWhirter, E ;

Handelsman, MYC ;

Brunton, JL ;

Sherman, PM .

INFECTION AND IMMUNITY, 1998, 66 (04) :1688-1696

[9] ENTEROPATHOGENIC ESCHERICHIA-COLI CONTAINS A PUTATIVE TYPE-III SECRETION SYSTEM NECESSARY FOR THE EXPORT OF PROTEINS INVOLVED IN ATTACHING AND EFFACING LESION FORMATION [J].

JARVIS, KG ;

GIRON, JA ;

JERSE, AE ;

MCDANIEL, TK ;

DONNENBERG, MS ;

KAPER, JB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (17) :7996-8000

[10] PROTEIN SECRETION BY ENTEROPATHOGENIC ESCHERICHIA-COLI IS ESSENTIAL FOR TRANSDUCING SIGNALS TO EPITHELIAL-CELLS [J].

KENNY, B ;

FINLAY, BB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (17) :7991-7995