TEM-121, a novel complex mutant of TEM-type β-lactamase from Enterobacter aerogenes

被引：29

作者：

Poirel, L ^{[1
]}

Mammeri, H ^{[1
]}

Nordmann, P ^{[1
]}

机构：

[1] Univ Paris 11, Hop Bicetre, Assistance Publ Hop Paris, Fac Med Paris Sud,Serv Bacteriol Virol, F-74275 Le Kremlin Bicetre, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2004年 / 48卷 / 12期

关键词：

D O I：

10.1128/AAC.48.12.4528-4531.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enterobacter aerogenes clinical isolate LOR was resistant to penicillins and ceftazidime but susceptible to cefuroxime, cephalothin, cefoxitin, cefotaxime, ceftriaxone, and cefepime. PCR and cloning experiments from this strain identified a novel TEM-type beta-lactamase (TEM-121) differing by five amino acid substitutions from beta-lactamase TEM-2 (Glu104Lys, Arg164Ser, Ala237Thr, GIu240Lys, and Arg244Ser) and by only one amino acid change from the extended-spectrum beta-lactamase (ESBL) TEM-24 (Arg244Ser), with the last substitution also being identified in the inhibitor-resistant beta-lactamase IRT-2. Kinetic parameters indicated that TEM-121 hydrolyzed ceftazidime and aztreonam (like TEM-24) and was inhibited weakly by clavulanic acid and strongly by tazobactam. Thus, TEM-121 is a novel complex mutant TEM beta-lactamase (CMT-4) combining the kinetic properties of an ESBL and an inhibitor-resistant TEM enzyme.

引用

收藏

页码：4528 / 4531

页数：4

相关论文

共 22 条

[1] TEM-103/IRT-28 β-lactamase, a new TEM variant produced by Escherichia coli BM4511 [J].

Alonso, R ;

Gerbaud, G ;

Galimand, M ;

Courvalin, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (11) :3627-3629

[2] A STANDARD NUMBERING SCHEME FOR THE CLASS-A BETA-LACTAMASES [J].

AMBLER, RP ;

COULSON, AFW ;

FRERE, JM ;

GHUYSEN, JM ;

JORIS, B ;

FORSMAN, M ;

LEVESQUE, RC ;

TIRABY, G ;

WALEY, SG .

BIOCHEMICAL JOURNAL, 1991, 276 :269-270

[3] ESSENTIAL STRUCTURE OF ESCHERICHIA-COLI PROMOTER - EFFECT OF SPACER LENGTH BETWEEN THE 2 CONSENSUS SEQUENCES ON PROMOTER FUNCTION [J].

AOYAMA, T ;

TAKANAMI, M ;

OHTSUKA, E ;

TANIYAMA, Y ;

MARUMOTO, R ;

SATO, H ;

IKEHARA, M .

NUCLEIC ACIDS RESEARCH, 1983, 11 (17) :5855-5864

[4] Molecular and biochemical heterogeneity of class B carbapenem-hydrolyzing β-lactamases in Chryseobacterium meningosepticum [J].

Bellais, S ;

Aubert, D ;

Naas, T ;

Nordmann, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (07) :1878-1886

[5] Molecular characterization of TEM-59 (IRT-17), a novel inhibitor-resistant TEM-derived β-lactamase in a clinical isolate of Klebsiella oxytoca [J].

Bermudes, H ;

Jude, F ;

Chaibi, EB ;

Arpin, C ;

Bebear, C ;

Labia, R ;

Quentin, C .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (07) :1657-1661

[6] Tazobactam is a potent inactivator of selected inhibitor-resistant class A beta-lactamases [J].

Bonomo, RA ;

Rudin, SA ;

Shlaes, DM .

FEMS MICROBIOLOGY LETTERS, 1997, 148 (01) :59-62

[7] Most Enterobacter aerogenes strains in France belong to a prevalent clone [J].

Bosi, C ;

Davin-Regli, A ;

Bornet, C ;

Mallea, M ;

Pages, JM ;

Bollet, C .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (07) :2165-2169

[8] Inhibitor-resistant TEM β-lactamases:: phenotypic, genetic and biochemical characteristics [J].

Chaïbi, EB ;

Sirot, D ;

Paul, G ;

Labia, R .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1999, 43 (04) :447-458

[9] NUCLEOTIDE-SEQUENCES OF CAZ-2, CAZ-6, AND CAZ-7 BETA-LACTAMASE GENES [J].

CHANAL, C ;

POUPART, MC ;

SIROT, D ;

LABIA, R ;

SIROT, J ;

CLUZEL, R .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1992, 36 (09) :1817-1820

[10]

Cornish-Bowden A., 1995, Fundamentals of Enzyme Kinetics, P30