Metabolic flux analysis in mammalian cell culture

被引：155

作者：

Quek, Lake-Ee ^{[1
]}

Dietmair, Stefanie ^{[1
]}

Kroemer, Jens O. ^{[1
]}

Nielsen, Lars K. ^{[1
]}

机构：

[1] Univ Queensland, AIBN, Brisbane, Qld 4072, Australia

来源：

METABOLIC ENGINEERING | 2010年 / 12卷 / 02期

关键词：

Metabolic flux analysis (MFA); Mammalian cells; Review; Constrained-based analysis; Hybridoma; CHO; RECOMBINANT MONOCLONAL-ANTIBODY; MAGNETIC-RESONANCE SPECTROSCOPY; BIOCHEMICAL REACTION SYSTEMS; LINEAR CONSTRAINT RELATIONS; YEAST PYRUVATE-CARBOXYLASE; FED-BATCH CULTURE; HYBRIDOMA CELLS; MYELOMA CELLS; GLUTAMINE-METABOLISM; MASS BALANCES;

D O I：

10.1016/j.ymben.2009.09.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Mammalian cell culture metabolism is characterized by glucoglutaminolysis, that is, high glucose and glutamine uptake combined with a high rate of lactate and non-essential amino acid secretion. Stress associated with acid neutralization and ammonia accumulation necessitates complex feeding schemes and limits cell densities achieved in fed-batch culture. Conventional and constraint-based metabolic flux analysis has been successfully used to study the metabolic phenotype of mammalian cells in culture, while C-13 tracer analysis has been used to study small network models and validate assumptions of metabolism. Large-scale C-13 metabolic flux analysis, which is required to improve confidence in the network models and their predictions, remains a major challenge. Advances in both modeling and analytical techniques are bringing this challenge within sight. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：161 / 171

页数：11

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