An electrogenerated chemiluminescence probe and its application utilizing Tris(2,2'-bipyridyl)ruthenium(II) and luminol chemiluminescence without a flowing stream

被引:33
作者
Preston, JP [1 ]
Nieman, TA [1 ]
机构
[1] UNIV ILLINOIS,DEPT CHEM,URBANA,IL 61801
关键词
D O I
10.1021/ac9509162
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel electrogenerated chemiluminescent (ECL) probe that does not require a dark box to exclude ambient light has been developed. The probe is placed within the sample solution in a fashion similar to the use of a pH electrode. Thus, the flowing stream method of analyte introduction typically used for ECL analysis is not necessary. A fiber-optic bundle is utilized to carry the light produced within the probe to a photomultiplier tube for detection. Within the body of this probe is a three-electrode configuration necessary for ECL generation. The three ECL systems, luminol, tris(2,2'-bipyridyl)ruthenium(III), and luminol coupled with an immobilized oxidase enzyme, have been used with this probe. The relative standard deviation for a typical sample was found to be 0.6%, Glucose determinations ranging from 3.0 to 1000 mu M were made without a pH change for several real samples employing luminol and glucose oxidase immobilized within the probe body.
引用
收藏
页码:966 / 970
页数:5
相关论文
共 24 条
[1]  
Bard A. J., 1980, ELECTROCHEMICAL METH
[2]   ROLE OF ELECTRON-DONATING WITHDRAWING CHARACTER, PH, AND STOICHIOMETRY ON THE CHEMILUMINESCENT REACTION OF TRIS(2,2'-BIPYRIDYL)RUTHENIUM(III) WITH AMINO-ACIDS [J].
BRUNE, SN ;
BOBBITT, DR .
ANALYTICAL CHEMISTRY, 1992, 64 (02) :166-170
[3]  
ENGSTROM RC, 1991, J ELECTROCHEM SOC, V138, P1637
[4]   ELECTROCHEMICAL GENERATION OF SOLUTION LUMINESCENCE .3. EFFECT OF OXYGEN ON QUANTUM YIELD AND KINETICS OF LUMINOL [J].
EPSTEIN, B ;
KUWANA, T .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1967, 6 (09) :605-&
[5]   ELECTROOXIDATION OF PHTHALHYDRAZIDES [J].
EPSTEIN, B ;
KUWANA, T .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1967, 15 (04) :389-&
[6]  
EPSTEIN B, 1965, PHOTOCHEM PHOTOBIOL, V4, P1157
[7]   CHEMILUMINESCENCE DETECTION OF AMINO-ACIDS, PEPTIDES, AND PROTEINS USING TRIS-2,2'-BIPYRIDINE RUTHENIUM(III) [J].
HE, L ;
COX, KA ;
DANIELSON, ND .
ANALYTICAL LETTERS, 1990, 23 (02) :195-210
[8]   LIQUID-CHROMATOGRAPHY OF ANTIHISTAMINES USING POSTCOLUMN TRIS(2,2'-BIPYRIDINE) RUTHENIUM(III) CHEMILUMINESCENCE DETECTION [J].
HOLEMAN, JA ;
DANIELSON, ND .
JOURNAL OF CHROMATOGRAPHY A, 1994, 679 (02) :277-284
[9]   MODULATED POTENTIAL ELECTROGENERATED CHEMILUMINESCENCE OF LUMINOL AND RU(BPY)3(2+) [J].
JIRKA, GP ;
NIEMAN, TA .
MIKROCHIMICA ACTA, 1994, 113 (3-6) :339-347
[10]  
JIRKA GP, 1993, ANAL CHIM ACTA, V284, P337