Golgin-84 is a Rab1 binding partner involved in Golgi structure

被引：107

作者：

Satoh, A ^{[1
]}

Wang, Y ^{[1
]}

Malsam, J ^{[1
]}

Beard, MB ^{[1
]}

Warren, G ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06520 USA

来源：

TRAFFIC | 2003年 / 4卷 / 03期

关键词：

cisterna; Golgi; golgin; GTPase; mitosis; rab;

D O I：

10.1034/j.1600-0854.2003.00103.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.

引用

页码：153 / 161

页数：9

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