Polycomb complexes act redundantly to repress genomic repeats and genes

被引：264

作者：

Leeb, Martin ^{[1
]}

Pasini, Diego ^{[2
,3
]}

Novatchkova, Maria ^{[1
]}

Jaritz, Markus ^{[1
]}

Helin, Kristian ^{[2
,3
]}

Wutz, Anton ^{[1
]}

机构：

[1] Res Inst Mol Pathol, A-1030 Vienna, Austria

[2] Univ Copenhagen, BRIC, DK-2200 Copenhagen, Denmark

[3] Univ Copenhagen, Ctr Epigenet, DK-2200 Copenhagen, Denmark

来源：

GENES & DEVELOPMENT | 2010年 / 24卷 / 03期

基金：

英国惠康基金; 奥地利科学基金会;

关键词：

Polycomb; histone modification; chromatin; embryonic stem cells; tumor; retrovirus; EMBRYONIC STEM-CELLS; HISTONE METHYLTRANSFERASE ACTIVITY; GROUP PROTEINS RING1A/B; X-INACTIVATION; RESPONSE ELEMENTS; CHROMATIN; H3; METHYLATION; H2A; PLURIPOTENCY;

D O I：

10.1101/gad.544410

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Polycomb complexes establish chromatin modifications for maintaining gene repression and are essential for embryonic development in mice. Here we use pluripotent embryonic stem (ES) cells to demonstrate an unexpected redundancy between Polycomb-repressive complex 1 (PRC1) and PRC2 during the formation of differentiated cells. ES cells lacking the function of either PRC1 or PRC2 can differentiate into cells of the three germ layers, whereas simultaneous loss of PRC1 and PRC2 abrogates differentiation. On the molecular level, the differentiation defect is caused by the derepression of a set of genes that is redundantly repressed by PRC1 and PRC2 in ES cells. Furthermore, we find that genomic repeats are Polycomb targets and show that, in the absence of Polycomb complexes, endogenous murine leukemia virus elements can mobilize. This indicates a contribution of the Polycomb group system to the defense against parasitic DNA, and a potential role of genomic repeats in Polycomb-mediated gene regulation.

引用

页码：265 / 276

页数：12

共 63 条

[1] The Polycomb-associated protein Rybp is a ubiquitin binding protein [J].