An investigation into the influence of secondary structures on DNA hybridization using surface plasmon resonance biosensing

被引：17

作者：

Chien, FC

Liu, JS

Su, HJ

Kao, LA

Chiou, CF

Chen, WY

Chen, SJ ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 701, Taiwan

[2] Natl Cent Univ, Inst Opt Sci, Chungli 320, Taiwan

[3] Natl Cent Univ, Dept Chem & Mat Engn, Chungli 320, Taiwan

[4] Ind Technol Res Inst, Ctr Biomed Engn, Hsinchu 300, Taiwan

[5] Phalanx Biotech Inc, Hsinchu 300, Taiwan

来源：

CHEMICAL PHYSICS LETTERS | 2004年 / 397卷 / 4-6期

关键词：

D O I：

10.1016/j.cplett.2004.09.007

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This study utilizes a surface plasmon resonance (SPR) biosensing and a theoretical secondary structure calculation to investigate the influence of secondary structures on the DNA hybridization. It is found that the SPR angular shifts associated with the three pairs of 60mer oligonucleotides with prominent secondary structures are lower than those observed for the two pairs of oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35 to 45 degreesC reduces secondary structure effects. On the hybridization with mixture target oligonucleotides, the SPR results demonstrate that secondary structures interfere significantly. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：429 / 434

页数：6

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