A large-scale in situ hybridization system using an equalized cDNA library

被引：25

作者：

Komiya, T ^{[1
]}

Tanigawa, Y ^{[1
]}

Hirohashi, S ^{[1
]}

机构：

[1] NATL CANC CTR,RES INST,DIV PATHOL,CHUO KU,TOKYO 104,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1997年 / 254卷 / 01期

关键词：

D O I：

10.1006/abio.1997.2399

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a large-scale in situ hybridization system in which all the procedures are carried out on a 96-well format: digoxigenin-labeled probes were synthesized from PCR-amplified templates, sections were mounted on 96-well plates, and hybridization and immunohistochemistry protocols were carried out in each well of the plates. This system in combination with equalized (normalized) cDNA library as the source for the probes enables us to identify the cellular distribution of many mRNAs in various tissues rapidly and efficiently, Thus, this system may be a novel cloning method of identify genes differentially expressed in many tissues, In addition, this system has a potential to be automated. (C) 1997 Academic Press.

引用

页码：23 / 30

页数：8

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