Simultaneous variation of temperature and gradient steepness for reversed-phase high-performance liquid chromatography method development - I. Application to 14 different samples using computer simulation

被引:91
作者
Dolan, JW
Snyder, LR
Djordjevic, NM
Hill, DW
Saunders, DL
Van Heukelem, L
Waeghe, TJ
机构
[1] LC Resources Inc, Walnut Creek, CA 94596 USA
[2] Novartis Pharma AG, CH-4002 Basel, Switzerland
[3] Univ Connecticut, Microchem Lab, Storrs, CT 06269 USA
[4] Avery Dennison, Pasadena, CA 91107 USA
[5] UMCES, Horn Point Lab, Cambridge, MD 21613 USA
[6] Dupont Co, Expt Stn, Wilmington, DE 19880 USA
基金
美国国家卫生研究院;
关键词
computer simulation temperature effects; gradient elution; optimization; resolution; method development; steroids; pesticides; algal pigments; fatty acid methyl esters; acrylate monomers;
D O I
10.1016/S0021-9673(97)01293-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The optimized reversed-phase HPLC separation of 14 different samples is reported, based on simultaneous changes in temperature and gradient steepness. Four experimental runs are required for each sample, following which preferred conditions can be predicted using computer simulation software (DryLab). The overall accuracy and effectiveness of this method development approach is discussed, with particular attention to the use of resolution maps provided by the software. These maps are useful for maximizing resolution for the total sample, for optimizing the separation of a smaller number of selected sample compounds, and as an initial step in the separation of more demanding samples. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:1 / 31
页数:31
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