Measurement of natural (CD4+CD25high) and inducible (CD4+IL-10+) regulatory T cells in patients with systemic lupus erythematosus

被引:64
作者
Barath, S. [1 ]
Aleksza, M. [1 ]
Tatt, T. [1 ]
Sipka, S. [1 ]
Szegedi, G. [1 ]
Kiss, E. [1 ]
机构
[1] Univ Debrecen, Med & Hlth Sci Ctr, Dept Internal Med 3, H-4032 Debrecen, Hungary
关键词
CD4(+)CD25(high); foxp3(+); constitutional; IL-10; inducible; regulatory T cells; SLE;
D O I
10.1177/0961203307080226
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Abnormalities of regulatory T cells may play an important role in the loss of self-tolerance, which is a major characteristic of lupus. The objective of this study was to determine the ratio and the number of natural CD4(+)CD25(high)Foxp3(+) and inducible CD4(+)IL-10(+) regulatory T cells in lupus patients and to search correlation with disease activity. Seventy-two Hungarian lupus patients were enrolled in the study. Fourty-one age- and sex matched healthy donors served as controls. Flow cytometry was used for the quantification of CD4(+)CD25(high) Foxp3(+) (nTreg) and CD4(+)IL-10(+) (iTreg) cells. The ratio (3.06 +/- 1.45%) and the number (0.019 +/- 0,012 X 10(9)/L) of nTreg cells decreased it) lupus significantly (P < 0.001 in both) as compared to normal controls (4.26 +/- 1.01% and 0.039 +/- 0.017 X 10(9)/L). The ratio of iTreg cells were significantly higher in patients than in controls (20.92 +/- 14.02% versus 15.49 +/- 11.65%, P < 0.03), but the number of these cell type did not differ in significant manner (0.314 +/- 0.236 X 10(9)/L- versus 0.259 +/- 0.183 X 10(9)/L). The 19 active patients were characterised by significantly higher disease activity index (SLEDAI 8.63 +/- 2.95 versus 1.74 +/- 1.68, P < 0.001) and anti-DNA concentration (117.85 +/- 145.89 versus 37.36 - 68.851U/mL, P = 0.001) as compered to the 52 inactive patients. Furthermore, active patients required higher dose of methylprednisolon than inactive ones (14.8 +/- 10.6 versus 4.8 +/- 3.4 mg/day, P < 0.00 1). However, we did not find statistical significant difference in the number and ratio of the examined cell populations regarding to disease activity. Altered ratio and number of both natural and inducible regulatory T cells may play a role in the pathogenesis of lupus. There are small but appreciable difference in the number of regulatory T cells between inactive patients and healthy controls. It suggests that immunoregulatory deficiencies are present in the inactive stage of the disease also.
引用
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页码:489 / 496
页数:8
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