A comparative study of clinically well-characterized human atherosclerotic plaques with histological, chemical, and ultrastructural methods

被引:51
作者
Becker, A
Epple, M
Müller, KM
Schmitz, I
机构
[1] Ruhr Univ Bochum, Inst Pathol & German Mesothelioma Register, Bergmannsheil Clin, D-44789 Bochum, Germany
[2] Univ Duisburg Gesamthsch, Inst Inorgan Chem, D-45111 Essen, Germany
关键词
atherosclerosis; calcification; electron rnicroscopy; IR-spectroscopy; X-ray diffraction;
D O I
10.1016/j.jinorgbio.2004.09.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Atherosclerotic plaques (six cases) with well-documented clinical history were analysed using histology, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray (EDX) spectroscopy, infrared spectroscopy (IR), thermogravimetry (TG), and high -resolution synchrotron X-ray diffraction. All samples contained about 60-70 wt% biological carbonated apatite (in dry state) in a nanocrystalline form with particle sizes of about 20 nm. Structurally, there are strong similarities to bone mineral. Ultrastructural investigations documented typical calcospherites, mineralisation processes starting at collagen fibrils and ring-shaped crystalline mineralised structures. There were no significant ultrastructural or chemical differences between the calcifications of individual patients. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:2032 / 2038
页数:7
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