A simple voltage controlled enzymatic nanoreactor produced in the tip of a nanopipet

被引：13

作者：

Bruckbauer, A ^{[1
]}

Zhou, DJ ^{[1
]}

Ying, LM ^{[1
]}

Abell, C ^{[1
]}

Klenerman, D ^{[1
]}

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

NANO LETTERS | 2004年 / 4卷 / 10期

关键词：

D O I：

10.1021/nl049029h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the enzymatic cleavage of fluorescein diphosphate (FDP) by alkaline phosphatase (AP) confined in the tip of a nanopipet with a volume of approximately 100 attoliters. The amount of enzyme that reversibly adsorbs in the pipet tip was shown to be proportional to the enzyme concentration in the bath. Increasing the voltage applied to the pipet, up to 1.5 V, linearly controls the substrate turnover rate by increasing the flow of substrate out of the pipet. This work opens up possibilities of highly miniaturized and sensitive enzyme assays with high spatial resolution.

引用

页码：1859 / 1862

页数：4

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