Fingerprint analysis of the fruits of Cnidium monnieri extract by high-performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry

被引：44

作者：

Chen, Yi

Fan, Guorong

Zhang, Qiaoyan

Wu, Huiling

Wu, Yutian

机构：

[1] Second Mil Med Univ, Sch Pharm, Dept Pharmaceut Anal, Shanghai 200433, Peoples R China

[2] Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China

[3] Second Mil Med Univ, Sch Pharm, Dept Pharmacognosy, Shanghai 200433, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2007年 / 43卷 / 03期

关键词：

HPLC-DAD-ESI-MS/MS; coumarin; the fruits of Cnidium monnieri; fingerprint analysis;

D O I：

10.1016/j.jpba.2006.09.015

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method incorporating high-performance liquid chromatography (HPLC) with electrospray ionization (ESI) and tandem mass spectrometry (MS), with parallel analysis by HPLC with UV detection using a diode-array detector (DAD) was developed for the qualitative characterization of coumarin and chromone constituents in the fruits of Cnidium monnieri. The chromatographic separations were performed on a Diamonsil (TM) C18 column (4.6 mm x 200 mm, 5 mu m) with water with 50 mM ammonium acetate and 2% acetic acid (A) and acetonitrile (B) as the mobile phase. According to the characteristic UV spectra, the information of molecular weight and structure provided by ESI-MS/MS, 13 coumarin and 7 chromone components were detected and identified. This method is rapid and reliable for identification of the constituents in the complex herbal system, and the fragmentation patterns proposed could be extended to the similar compounds. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：926 / 936

页数：11

共 55 条

[1] Prenylated flavonoids, monoterpenoid furanocoumarins and other constituents from the twigs of Dorstenia elliptica (Moraceae) [J].