Hydrolysis of rice bran oil using immobilized lipase in a stirred batch reactor

被引：10

作者：

Murty V.R. ^{[1
]}

Bhat J. ^{[1
]}

Muniswaran P.K.A. ^{[1
]}

机构：

[1] Department of Chemical Engineering, Manipal Institute of Technology, Deemed University

来源：

Biotechnology and Bioprocess Engineering | 2002年 / 7卷 / 6期

关键词：

Degree of hydrolysis and chemical equilibrium; Free fatty acids; Immobilized lipase; Protein loading;

D O I：

10.1007/BF02933523

中图分类号：

学科分类号：

摘要：

Candida cylindracea lipase was immobilized by adsorption on acid washed glass beads. It was observed that protein loading of the support depends on the size of the particle, with smaller particle containing higher amount of protein per unit weight. Initial reaction rate linearly varied up to enzyme concentration of 17.25 U/mL. Amount of free fatty acids produced was linearly proportional up to the enzyme loading of 1650 μg/g of bead. Achievement of chemical equilibrium took longer time in the case of less protein loading. Degree of hydrolysis was found to decrease in second and third consecutive batch operations on repeated use of immobilized lipase.

引用

页码：367 / 370

页数：3

共 18 条

[1]

Kosugi Y., Suzuki H., Funada T., Hydrolysis of beef tallow by lipase from Pseudomonas sp, Biotechnol. Bioeng., 31, pp. 349-356, (1988)

[2]

Wang Y.J., Sheu J.Y., Wang F.F., Shaw J.F., Lipase catalyzed oil hydrolysis in the absence of added emulsifier, Biotechnol. Bioeng., 31, pp. 628-633, (1988)

[3]

Hoq M.M., Yamane T., Shimizu S., Funada T., Ishida S., Continuous hydrolysis of olive oil by lipase in microporous hydrophobic membrane bioreactor, J. Am. Oil Chem. Soc., 62, pp. 1016-1021, (1985)

[4]

Khor H.T., Tan N.H., Chua C.L., Lipase catalyzed hydrolysis of palm oil, J. Am. Oil Chem. Soc., 63, pp. 538-540, (1986)

[5]

Murty V.R.C., Bhat J., Muniswaran P.K.A., Hydrolysis of oils by using immobilized lipase enzyme: A review, Biotechnol. Bioprocess Eng., 7, pp. 57-66, (2002)

[6]

Kang S.T., Rhee J.S., Characteristics of immobilized lipase catalyzed hydrolysis of olive oil of high concentration in reverse phase system, Biotechnol. Bioeng., 33, pp. 1469-1476, (1989)

[7]

Tsai S.W., Chiang C.L., Kinetics, Mechanism, and time course analysis of lipase-catalyzed hydrolysis of high concentration of olive oil in AOT-isooctane reversed micelles, Biotechnol. Bioeng., 38, pp. 206-211, (1991)

[8]

Prazeres D.M.F., Garcia F.A.P., Cabral J.M.S., An ultrafiltration membrane bioreactor for the lipolysis of olive oil in reversed micellar media, Biotechnol. Bioeng., 41, pp. 761-770, (1993)

[9]

Yang D., Rhee J.S., Continuous hydrolysis of olive oil by immobilized lipase in organic solvent, Biotechnol. Bioeng., 40, pp. 748-752, (1992)

[10]

Yamane T., Hoq M.M., Shimizu S., Kinetics of continuous hydrolysis of olive oil by lipase in microporous hydrophobic membrane bioreactor, J. Jpn. Oil Chem. Soc., 35, pp. 10-17, (1986)

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