Biofilms of As(III)-oxidising bacteria: Formation and activity studies for bioremediation process development

被引：42

作者：

Michel C. ^{[1
]}

Jean M. ^{[1
]}

Coulon S. ^{[1
]}

Dictor M.-C. ^{[1
]}

Delorme F. ^{[1
]}

Morin D. ^{[1
]}

Garrido F. ^{[1
]}

机构：

[1] BRGM, Orléans Cedex 2 45060

来源：

Applied Microbiology and Biotechnology | 2007年 / 77卷 / 02期

关键词：

As(III)-oxidase; Biofilm; Bioremediation; EPS; Fixed-bed bioreactor; Thiomonas arsenivorans;

D O I：

10.1007/s00253-007-1169-4

中图分类号：

学科分类号：

摘要：

The formation and activity of an As(III)-oxidising biofilm in a bioreactor, using pozzolana as bacterial growth support, was studied for the purpose of optimising fixed-bed bioreactors for bioremediation. After 60 days of continuous functioning with an As(III)-contaminated effluent, the active biofilm was found to be located mainly near the inflow rather than homogeneously distributed. Biofilm development by the CAsO1 bacterial consortium and by Thiomonas arsenivorans was then studied both on polystyrene microplates and on pozzolana. Extra-cellular polymeric substances (EPS) and yeast extract were found to enhance bacteria attachment, and yeast extract also appears to increase the kinetics of biofilm formation. Analysis of proteins, sugars, lipids and uronic acids indicate that sugars were the main EPS components. The specific As(III)-oxidase activity of T. arsenivorans was higher (by ninefold) for planktonic cells than for sessile ones and was induced by As(III). All the results suggest that the biofilm structure is a physical barrier decreasing As(III) access to sessile cells and thus to As(III)-oxidase activity induction. The efficiency of fixed-bed reactors for the bioremediation of arsenic-contaminated waters can be thus optimised by controlling different factors such as temperature and EPS addition and/or synthesis to increase biofilm density and activity. © 2007 Springer-Verlag.

引用

页码：457 / 467

页数：10

共 45 条

[1]

Anderson G., Williams J., Hille R., The purification and characterization of arsenite oxidase from Alcaligenes faecalis, a molybdenum-containing hydroxylase, J Biol Chem, 267, pp. 23674-23682, (1992)

[2]

Battaglia-Brunet F., Dictor M.C., Garrido F., Crouzet C., Morin D., Dekeyser K., Clarens M., Baranger P., An As(III)-oxidizing bacterial population: Selection, characterization, and performance in bioreactors, J Appl Microbiol, 93, pp. 656-667, (2002)

[3]

Battaglia-Brunet F., Dictor M.C., Garrido F., Michel C., Joulian C., Bourgeois F., Baranger P., Itard Y., Morin D., Remediation processes using biological As(III) oxidation, Proceedings of the 16th International Biohydrometallurgy Symposium, pp. 343-350, (2005)

[4]

Battaglia-Brunet F., Joulian C., Garrido F., Dictor M.C., Morin D., Coupland K., Johnson D., Hallberg K., Baranger P., Oxidation of arsenite by Thiomonas strains and characterisation of Thiomonas arsenivorans sp. Nov, Antonie Van Leeuwenhoek, 89, pp. 99-108, (2006)

[5]

Battaglia-Brunet F., Itard Y., Garrido F., Delorme F., Crouzet C., Greffie C., Joulian C., A simple biogeochemical process removing arsenic from a mine drainage water, Geomicrobiol J, 23, pp. 201-211, (2006)

[6]

Blumenkrantz N., Asboe-Hansen G., New method for quantitative determination of uronic acids, Anal Biochem, 54, pp. 484-489, (1973)

[7]

Challan Belval S., Gal L., Margiewes S., Garmyn D., Piveteau P., Guzzo J., Assessment of the roles of LuxS, S-ribosyl homocysteine, and autoinducer 2 in cell attachment during biofilm formation by Listeria monocytogenes EGD-e, Appl Env Microbiol, 72, pp. 2644-2650, (2006)

[8]

Clifford D., Ghurye G., Tripp A., Abermathy R., Calderon R., Development of an anion exchange process for arsenic removal from water, Arsenic Exposure and Health Effect, pp. 379-387, (1999)

[9]

Costerton J., Introduction to biofilm, Int J Antimicrob Agents, 11, pp. 217-221, (1999)

[10]

Daniels L., Hanson R., Phillips J., Gerhardt P., Murray R.G., Wood W.A., Krieg N.R., Chemical analysis, Methods for General and Molecular Bacteriology, pp. 512-554, (1994)

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