Effect of molecular characteristics of DNA on its adsorption and binding on homoionic montmorillonite and kaolinite

被引：94

作者：

Pietramellara G. ^{[1
]}

Franchi M. ^{[2
]}

Gallori E. ^{[2
]}

Nannipieri P. ^{[1
]}

机构：

[1] Dipto. Sci. Suolo Nutrizione D., Università di Firenze, 50144, Firenze

[2] Dipto. di Biol. Animale e Genetica, Università di Firenze, 50120 Firenze

来源：

Biology and Fertility of Soils | 2001年 / 33卷 / 5期

关键词：

Adsorption; Binding sites; Clay edge surfaces; Clay planar surfaces; Clay-DNA complexes;

D O I：

10.1007/s003740100341

中图分类号：

学科分类号：

摘要：

Adsorption or binding of DNA by montmorillonite or kaolinite, homoionic to Ca2+, was not affected by base composition, blunt or cohesive ends. Fitting data to both Freundlich and Langmuir adsorption isotherms showed that the amount of lower molecular mass DNA adsorbed and bound by both clay minerals was higher than that of the higher molecular mass DNA. The relevance of phosphate groups for the adsorption of DNA by clay minerals was investigated by adding sodium metaphosphate before and after the addition of DNA to clay minerals: DNA was partially not adsorbed even at low concentrations of sodium metaphosphate. The fact that the observed DNA was partially desorbed by washing with double-distilled H2O indicated that bonds with different degrees of strength were formed between DNA molecules and clay minerals. The higher molecular mass DNA could interact with a larger number of binding sites on the external surface of clay mineral than the lower molecular mass DNA. The number of external surface binding sites was higher on kaolinite than on montmorillonite.

引用

页码：402 / 409

页数：7

共 28 条

[1]

Alvarez A.J., Khanna M., Toranzos G.A., Stotzky G., Amplification of DNA bound on clay minerals, Mol Ecol, 7, pp. 7755-7778, (1998)

[2]

Arderma B.W., Lorenz M.G., Krumbein W.E., Protection of sediment adsorbed transforming DNA against enzymatic inactivation, Appl Environ Microbiol, 46, pp. 417-420, (1983)

[3]

Boeynaems J.M., Dumont J.E., Quantitative analysis of the binding of ligands to their receptors, J Cycl Nucl Res, 1, pp. 123-142, (1975)

[4]

Cortez J., Schnitzer M., Reactions of nucleic acid bases with inorganic soil constituents, Soil Biol Biochem, 13, pp. 173-178, (1981)

[5]

Crecchio C., Stotzky G., Binding of DNA on humic acids: Effect on transformation of Bacillus subtilis and resistance to DNase, Soil Biol Biochem, 30, pp. 1061-1067, (1998)

[6]

Davis R.W., Bostein D., Roth J.R., Advanced Bacterial Genetics, (1980)

[7]

Franchi M., Bramanti E., Morassi Bonzi E., Orioli P.E., Vettori C., Gallori E., Clay-nucleic acid complexes: Characteristics and implications for the preservation of genetic material in primeval habitats, Origins Life Evol Biosphere, 29, pp. 297-315, (1999)

[8]

Fusi P., Ristori G., Calamai E., Stotzky G., Adsorption and binding of protein on "clean" (homoionic) and "dirty" (coated with Fe oxyhydroxides) montmorillonite, illite, and kaolinite, Soil Biol Biochem, 21, pp. 911-920, (1989)

[9]

Gallori E., Bazzicalupo M., Dal Canto L., Fani R., Nannipieri P., Vettori C., Stotzky G., Transformation of Bacillus subtilis by DNA bound on clay minerals in non-sterile soil, FEMS Microbiol Ecol, 15, pp. 119-126, (1994)

[10]

Goring C.A.I., Bartholomew W.V., Adsorption of mononucleotides, nucleic acids, and nucleoproteins by clays, Soil Sci, 74, pp. 149-164, (1952)

← 1 2 3 →