Identification of a BIBAC clone that co-segregates with the petunia restorer of fertility (RF) gene

被引：20

作者：

Bentolila S. ^{[1
]}

Hanson M.R. ^{[1
]}

机构：

[1] Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca

来源：

Molecular Genetics and Genomics | 2001年 / 266卷 / 2期

基金：

美国农业部; 美国国家卫生研究院;

关键词：

Binary BACs (BIBACs); Fertility restorer; Petunia; Pollen development; Positional cloning;

D O I：

10.1007/s004380100544

中图分类号：

学科分类号：

摘要：

Molecular markers closely linked to the Restorer of fertility (Rf) locus in petunia were sought by conducting a bulk segregant analysis. The co-segregation of markers and Rf was tested on a large BC1 population produced from two different parental lines carrying Rf- The recombination frequency between OP704 and ECCA/MACT, the two most distal markers utilized in the fine-scale mapping, was significantly different in populations derived from parents that carry different nuclear backgrounds. The fine mapping identified an amplified fragment length polymorphism (AFLP) marker that co-segregates with Rf. A petunia BIBAC library (four genome equivalents), with an average insert size of 70 kb, was constructed and screened with the linked marker. A contiguous map was constructed from three different BIBAC clones that hybridized to the marker. As a result, we have identified a 37.5-kb BIBAC clone that co-segregates with Rf.

引用

页码：223 / 230

页数：7

共 27 条

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