Bacterial membrane injuries induced by lactacin F and nisin

被引：17

作者：

Dalmau M. ^{[1
]}

Maier E. ^{[2
]}

Mulet N. ^{[1
]}

Viñas M. ^{[1
]}

Benz R. ^{[2
]}

机构：

[1] Biomedical Research Center of Bellvitge, Laboratory of Microbiology, University of Barcelona

[2] Lehrstuhl für Biotechnologie, Theodor-Boveri-Institut (Biozentrum), Universität Würzburg, 97074 Würzburg, Am Hubland

来源：

International Microbiology | 2002年 / 5卷 / 2期

关键词：

Flow cytometry; Lactacin F; Lactic acid bacteria (LAB); Nisin; Planar lipid bilayer;

D O I：

10.1007/s10123-002-0063-2

中图分类号：

学科分类号：

摘要：

The combined action of nisin and lactacin F, two bacteriocins produced by lactic acid bacteria, is additive. In this report, the basis of this effect is examined. Channels formed by lactacin F were studied by experiments using planar lipid bilayers, and bactericidal effects were analyzed by flow cytometry. Lactacin F produced pores with a conductance of 1 ns in black lipid bilayers in 1 mM KCl at 10 mV at 20 °C. Pore formation was strongly dependent on voltage. Although lactacin F formed pores at very low potential (10 mV), the dependence was exponential above 40 mV. The injuries induced by nisin and lactacin F in the membranes of Lactobacillus helveticus produced different flow cytometric profiles. Probably, when both bacteriocins are present, each acts separately; their cooperation may be due to an increase in the number of single membrane injuries. © Springer-Verlag and SEM 2002.

引用

页码：73 / 80

页数：7

共 34 条

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