Expression of the mouse histone gene H1t begins at premeiotic stages of spermatogenesis

被引：42

作者：

Drabent B. ^{[1
]}

Bode C. ^{[1
]}

Miosge N. ^{[2
]}

Herken R. ^{[2
]}

Doenecke D. ^{[1
]}

机构：

[1] Division of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 Göttingen

[2] Division of Histology, Department of Anatomy, Georg-August Univ. Göttingen

来源：

Cell and Tissue Research | 1997年 / 291卷 / 1期

关键词：

Histone H1t; Immunogold staining; In situ hybridization; Mouse (NMRI); Spermatogenesis; Western blot analysis;

D O I：

10.1007/s004410050986

中图分类号：

学科分类号：

摘要：

The gene encoding H1t, a testicular variant of histone H1, is expressed in mammals during spermatogenesis. Northern blot and in situ hybridization has detected H1t mRNA only at the stage of pachytene spermatocytes. We have extended this analysis to more sensitive approaches and demonstrate, by RNase protection and electron-microscopic in situ hybridization, that H1t mRNA is detectable even in spermatogonia. Just a faint H1t bond is seen in Western blots of nuclear protein from 9-day-old mice. This indicates that the H1t gene is expressed at premeiotic stages, albeit at a low level. In contrast to H1t mRNA, the H1t protein has not been detected in spermatogonia by electron microscopy after immunogold staining.

引用

页码：127 / 132

页数：5

共 30 条

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