Differentiation from embryonic stem cells to vascular wall cells under in vitro pulsatile flow loading

被引：57

作者：

Huang H. ^{[1
]}

Nakayama Y. ^{[1
]}

Qin K. ^{[2
]}

Yamamoto K. ^{[2
]}

Ando J. ^{[2
]}

Yamashita J. ^{[3
]}

Itoh H. ^{[4
]}

Kanda K. ^{[5
]}

Yaku H. ^{[5
]}

Okamoto Y. ^{[6
]}

Nemoto Y. ^{[6
]}

机构：

[1] Department of Bioengineering, Advanced Medical Engineering Center, National Cardiovascular Center Research Institute, Suita, Osaka 565-8565

[2] Department of Biomedical Engineering, Graduate School of Medicine, University of Tokyo, Tokyo

[3] Laboratory of Stem Cell Differentiation, Institute for Frontier Medical Science, Kyoto University, Kyoto

[4] Department of Medicine and Clinical Science, Graduate School of Medicine, Kyoto University, Kyoto

[5] Second Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto

[6] Chemical Products Development Department, Bridgestone Corporation, Kanagawa

来源：

Journal of Artificial Organs | 2005年 / 8卷 / 2期

关键词：

Artificial vascular graft; Embryonic stem cell; Physical differentiation; Pulsatile flow; Vascular cell;

D O I：

10.1007/s10047-005-0291-2

中图分类号：

学科分类号：

摘要：

This study evaluated the possibility of differentiation from embryonic stem (ES) cells to vascular wall cells by physical (mechanical) stress loading in vitro. A cell mixture containing Flk1-positive cells (ca. 30%) derived from murine ES cells was added to a compliant microporous tube made of segmented polyurethane. The compliance of the tube was close to that of the human artery [the stiffness parameter (β) = 57.2 (n = 5, SD < 5%)]. The luminal surface of the tube was fully covered with the cells by preincubation for two days in the presence of vascular endothelial growth factor (VEGF). After 2 days of additional incubation without VEGF under static conditions, layering of the grown cells, mostly smooth muscle actin (SMA)-positive cells, was observed only on the luminal surface of the tube. The cells were flat, polygonal, and randomly oriented. On the other hand, after a 2-day incubation under a weak pulsatile flow simulating the human venous systems [wall shear stress (WSS) from -0.98 to 2.2 dyn/cm2; circumferential strain (CS) 4.6-9.6 × 10 4 dyn/cm2] without VEGF, cells in the superficial layer were regularly oriented in the direction of the pulsatial flow. The oriented cells exhibited endothelial-like appearance, indicating that they were platelet endothelial cell adhesion molecule 1 (PECAM1)-positive. In addition, the cells growing into the interstices in the deeper layer showed smooth muscle-like appearance, indicating that they were SMA-positive. Differentiation to two different cell types and segregation of incorporated ES cells may be simultaneously encouraged by the combination of WSS and CS. It is expected that the monobloc building of hierarchically structured hybrid vascular prostheses composed of several vascular wall cell types is possible by physically synchronized differentiation of ES cells. © The Japanese Society for Artificial Organs 2005.

引用

页码：110 / 118

页数：8

共 26 条

[1] Herring M., Gardner A., Glover J., A single-stage technique for seeding vascular graft with autologous endothelium, Surgery, 84, pp. 498-504, (1978)
[2] Weinberg C.B., Bell E., A blood vessel model constructed from collagen and vascular cells, Science, 231, pp. 397-400, (1986)
[3] Matsuda T., Miwa H., A hybrid vascular model biomimicking the hierarchic structure of arterial wall: Neointimal stability and neoarterial regeneration process under arterial circulation, J Thorac Cardiovasc Surg, 110, pp. 988-997, (1995)
[4] Noishiki Y., Yamane Y., Tomizawa Y., Okoshi T., Satoh S., Wildevuur C.R., Suzuki K., Rapid endothelialization of vascular prostheses by seeding autologous venous tissue fragments, J Thorac Cardiovasc Surg, 104, pp. 770-778, (1992)
[5] Naito Y., Imai Y., Shin'Oka T., Kashiwagi J., Successful clinical application of tissue-engineered graft for extracardiac fontan operation, J Thorac Cardiovasc Surg, 125, pp. 419-420, (2003)
[6] Karube N., Soma T., Noishiki Y., Yamazaki I., Kosuge T., Ichikawa Y., Takanashi Y., Clinical long-term results of vascular grafts sealed with fragmented autologous adipose tissue for aortoiliac occlusive disease, Artif Organs, 25, pp. 218-222, (2001)
[7] Yamashita J., Itoh H., Hirashima M., Ogawa M., Nishikawa S., Yurugi T., Naito M., Nakao K., Nishikawa S.I., Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors, Nature, 408, pp. 92-96, (2000)
[8] Yurugi-Kobayashi T., Itoh H., Yamashita J., Yamahara K., Hirai H., Kobayashi T., Ogawa M., Nishikawa S., Nishikawa S.I., Nakao K., Effective contribution of transplanted vascular progenitor cells derived from embryonic stem cells to adult neovascularization in proper differentiation stage, Blood, 101, pp. 2675-2678, (2003)
[9] Moore Jr. J.E., Burki E., Seciu A., Zhao S., Burnier M., Brunner H.R., Meister J.J., A device for subjecting vascular endothelial cells to both fluid shear stress and circumferential cyclic stretch, Ann Biomed Eng, 22, pp. 416-422, (1994)
[10] Kanda K., Matsuda T., Oka T., Mechanical stress induced cellular orientation and phenotypic modulation of 3-D cultured smooth muscle cells, ASAIO J, 39, pp. 686-690, (1993)

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