Time course of brain neuronal degeneration and heat shock protein (72) expression following neck tourniquet-induced cerebral ischemia in the rat

被引：16

作者：

Dasa Cizkova ^{[1
]}

Ivo Vanicky ^{[1
]}

Toshizo Ishikawa ^{[2
]}

Martin Marsala ^{[3
]}

机构：

[1] Institute of Neurobiology, SAS, 040 01 Kosice

[2] School of Allied Health Sciences, Yamaguchi University, Ube, Yamaguchi 755-8554

[3] Anesth. Research Laboratory-0818, University of California, San Diego, San Diego, CA 92093

来源：

Cellular and Molecular Neurobiology | 2000年 / 20卷 / 3期

关键词：

Cerebral ischemia; HSP72; expression; Neuronal degeneration; Silver impregnation;

D O I：

10.1023/A:1007018327133

中图分类号：

学科分类号：

摘要：

1. The present study was designed to examine the regional expression of HSP72/73 protein after a 7.5-min period of cerebral ischemia and to compare the distribution of HSP neurons with the localization of irreversible neuronal degeneration as analyzed by silver impregnation technique. 2. During 6-24 hr after cerebral ischemia clear-cut neuronal argyrophilia developed in several brain regions including the hippocampal hilus, nucleus reticularis thalami, and colliculi inferiores. With the exception of the hippocampal hilus, the structures which showed silver impregnability were HSP72 negative at 6-24 hr. 3. Despite the clear HSP72 expression seen in hippocampal CA1 neurons, a significant loss of these neurons was seen at 7 days after ischemia. 4. These data show that in some structures the presence of HSP72 is indicative of higher resistance of these neurons to ischemia-induced degeneration, however, the process of delayed neuronal degeneration appears to be independent of the accelerated synthesis of HSP72 seen during the early period of reflow.

引用

页码：367 / 381

页数：14

共 34 条

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