Micropropagation of American chestnut: Increasing rooting rate and preventing shoot-tip necrosis

被引：38

作者：

Xing Z. ^{[1
]}

Satchwell M.F. ^{[1
]}

Powell W.A. ^{[1
]}

Maynard C.A. ^{[1
]}

机构：

[1] State University of New York, Coll. of Environ. Sci. and Forestry, Faculty of Forestry, Syracuse, NY 13210

来源：

In Vitro Cellular & Developmental Biology - Plant | 1997年 / 33卷 / 1期

关键词：

Castanea dentata; Postrooting cultivation; Quick-dip; Shoot culture; Wounding;

D O I：

10.1007/s11627-997-0039-1

中图分类号：

学科分类号：

摘要：

A three-step medium sequence was developed for rooting microcuttings of American chestnut [Castanea dentata (Marsh.) Borkh.]. First, individual shoots or clumps of shoots were cultivated on shoot-elongation medium for 4-8 wk until shoots were 2-3 cm long. The medium consisted of modified Woody Plant Medium, 500 mg/l polyvinylpyrrolidone (MW 40,000), and 0.89 μM benzyladenine. Microcuttings were then excised, vertically split at the base to approximately 2 mm through the pith, dipped in 5 or 10 mM indolebutyric acid for 1 min, and cultivated on half-strength Murashige and Skoog basal medium plus 0.2 g/l charcoal for 2 wk. During that time, roots were induced and became visible. Finally, the microcuttings were transferred back to shoot-elongation medium and cultivated for 3 wk, allowing growth of both roots and shoots. Using this protocol with 3 genotypes derived from one mature and two 1-yr-old seedlings, 57 to 73% rooting was obtained with less than 23% shoot-tip necrosis.

引用

页码：43 / 48

页数：5

共 26 条

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