A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide

被引：79

作者：

Craig S. Nunemaker

R. Anthony DeFazio

Suzanne M. Moenter

机构：

[1] University of Virginia,Departments of Internal Medicine and Cell Biology, and the NSF Center for Biological Timing

[2] Virginia Commonwealth University,Department of Pharmacology and Toxicology

[3] R.W. Johnson Pharmaceuticals,Department of Internal Medicine

[4] University of Virginia,undefined

来源：

Biological Procedures Online | / 5卷 / 1期

关键词：

GnRH; rhythm; single-unit; neural network; loose-patch; extracellular;

D O I：

10.1251/bpo46

中图分类号：

学科分类号：

摘要：

Excitable cells in many endocrine and neuronal systems display rhythms with periodicities on the order of many minutes. To observe firing patterns that represent the output of these rhythms requires a recording technique that can monitor electrophysiological activity for several hours without affecting cell behavior. A targeted extracellular approach (also known as loose-patch) accomplishes this objective. Because low resistance seals (<20 MΩ) do not influence the cell membrane and because the normal intracellular milieu is maintained, this approach is the least invasive method for monitoring the endogenous electrical activity of single cells. In this report, we detail our use of this technique to record the firing patterns of gonadotropin-releasing hormone (GnRH) neurons in brain slices continuously for several hours.

引用

共 41 条

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