Effect of veratryl alcohol and manganese (IV) oxide on ligninolytic activity in semi solid cultures of Phanerochaete chrysosporium

被引：19

作者：

Couto S.R. ^{[1
]}

Rättö M. ^{[2
]}

机构：

[1] Department of Chemical Engineering, University of Vigo, E-36200 Vigo, Lagoas-Marcosende s/n

[2] VTT, Biotechnical Laboratory, SF-02150 Espoo

来源：

Biodegradation | 1998年 / 9卷 / 2期

关键词：

Carrier; LiP; Manganese (IV) oxide; MnP; Phanerochaete chrysosporium; Veratryl alcohol;

D O I：

10.1023/A:1008397913262

中图分类号：

学科分类号：

摘要：

An inert carrier (nylon sponge), a non-inert carrier (barley straw) and the addition of veratryl alcohol or manganese (IV) oxide to the cultures were used to study the production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) during semi solid state fermentation conditions. By supplementing the medium with these compounds we could stimulate the ligninolytic system of this fungus. The different carriers employed and the effect of adding veratryl alcohol or manganese (IV) oxide to the cultures were compared in order to determine the best system to produce high activities of ligninolytic enzymes. Lignin peroxidase (LiP) activities higher than 500 U/L and manganese-dependent peroxidase (MnP) activities about 1100 U/L were achieved.; An inert carrier (nylon sponge), a non-inert carrier (barley straw) and the addition of veratryl alcohol or manganese (IV) oxide to the cultures were used to study the production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) during semi solid state fermentation conditions. By supplementing the medium with these compounds we could stimulate the ligninolytic system of this fungus. The different carriers employed and the effect of adding veratryl alcohol or manganese (IV) oxide to the cultures were compared in order to determine the best system to produce high activities of ligninolytic enzymes. Lignin peroxidase (LIP) activities higher than 500 U/L and manganese-dependent peroxidase (MnP) activities about 1100 U/L were achieved.

引用

页码：143 / 150

页数：7

共 46 条

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