Study on transient expression of gus gene inChlorelia ellipsoidea (Chlorophyta) by using biolistic particle delivery system

被引：3

作者：

Chen Ying

Li Wen-bin

Bai Qin-hua

Sun Yong-ru

机构：

[1] Chinese Academy of Sciences,Institute of Genetics

来源：

Chinese Journal of Oceanology and Limnology | 1998年 / 16卷 / Suppl 1期

关键词：

gene transfer; transient expression; microprojectile bombardment; GUS gene;

D O I：

10.1007/BF02849079

中图分类号：

学科分类号：

摘要：

Study on the transient expression of GUS gene at different growing stage ofChlorella ellipsoidea using high velocity microprojectiles, the effects of osmosis, the distance between microprojectile and target cell, bombardment times, are reported in this paper. The results showed thatC. ellipsoidea in exponential phase has higer level of transient expression and that treatment with osmosis can improve the GUS transient expression notably. The effect of distance or bombardment times was not observed.

引用

页码：47 / 49

页数：2

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