Fine genetic mapping and BAC contig development for the citrus tristeza virus resistance gene locus in Poncirus trifoliata (Raf.)

被引：23

作者：

Deng Z. ^{[1
]}

Huang S. ^{[1
]}

Ling P. ^{[1
]}

Yu C. ^{[1
]}

Tao Q. ^{[2
]}

Chen C. ^{[1
]}

Wendell M. ^{[1
]}

Zhang H.-B. ^{[2
]}

Gmitter Jr F. ^{[1
]}

机构：

[1] University of Florida, Citrus Research and Education Center, Lake Alfred, FL 33850

[2] Texas A and M University, Department of Soil and Crop Sciences, Crop Biotechnology Center, College Station

来源：

Molecular Genetics and Genomics | 2001年 / 265卷 / 4期

关键词：

BAC contig development; Chromosome landing; Citrus tristeza virus (CTV); Genetic mapping; Resistance gene;

D O I：

10.1007/s004380100471

中图分类号：

学科分类号：

摘要：

A map-based cloning strategy has been employed to isolate Ctv, a single dominant gene from Poncirus trifoliata that confers resistance to citrus tristeza virus (CTV), the most important viral pathogen of citrus. Cloning of this gene will allow development of commercially acceptable, virus-resistant cultivars. A high-resolution genetic linkage map of the Ctv locus region was developed using a backcross population of 678 individuals. Three DNA markers that were closely linked or co-segregated with Ctv were identified and used to screen BAC libraries derived from an intergeneric hybrid of Poncirus and Citrus. Through chromosome walking and landing, two BAC contigs were developed: one encompassing the Ctv region, and the other spanning the allelic susceptibility gene region. The resistance gene contig consists of 20 BAC clones and is approximately 550 kb in length; the susceptibility gene contig consists of 16 BAC clones and extends about 450 kb. The Ctv locus was localized within a genomic region of approximately 180 kb by genetic mapping of BAC insert ends. The BAC contigs were integrated with the genetic map; variation in the ratio of genetic to physical distance was observed in the vicinity of Ctv. Southern hybridization data indicated that a few copies of NBS-LRR class sequences are distributed at or around the Ctv locus. Efforts are being made to assign the Ctv locus to a smaller genomic fragment whose function can be confirmed through genetic complementation of a CTV susceptible phenotype. These results indicate that map-based gene cloning is feasible in a woody perennial.

引用

页码：739 / 747

页数：8

共 40 条

[1]

Albiach-Marti M.R., Grosser J.W., Hilf M.E., Gowda S., Mawassi M., Satyanarayana T., Garnsey S.M., Dawson W.O., CTV-resistant plants are not immune to CTV at the cellular level, Annual Meeting of the American Society for Virology, (1999)

[2]

Arumuganathan K., Earle E., Nuclear DNA content of some important plant species, Plant Mol Biol Rep, 9, pp. 208-218, (1991)

[3]

Bent A.F., Kundel B.N., Dahlbeck D., Brown K.L., Schmidt R., Giraudat J., Leung J., Staskawicz B.J., RPS2 of Arabidopsis thaliana: A leucine-rich repeat class of plant disease resistance genes, Science, 265, pp. 1856-1860, (1994)

[4]

Buschges R., Hollricher K., Panstruga R., Simons G., Wolter M., Frijters A., Van Daelen R., Van der Lee T., Diergaarde P., Groenendijk J., Topsch S., Vos P., Salamini F., Schulze-Lefert P., The barley Mlo gene: A novel control element of plant pathogen resistance, Cell, 88, pp. 695-765, (1997)

[5]

Cai D.G., Kleine M., Kifle S., Harloff H.-J., Sandal N.N., Marcker K.A., Klein-Lankhorst R., Salentijn E.M.J., Lange W., Stiekema W.J., Wyss U., Grundler F.M.W., Jung C., Positional cloning of a gene for nematode resistance in sugar beet, Science, 275, pp. 832-834, (1997)

[6]

Danesh D., Penuela S., Mudge J., Denny R.L., Nordstrom H., Martinez J.P., Young N.D., A bacterial artificial chromosome library for soybean and identification of clones near a major cyst nematode resistance gene, Theor Appl Genet, 96, pp. 196-202, (1998)

[7]

Deng Z., Huang S., Xiao S., Gmitter F.G., Development and characterization of SCAR markers linked to the citrus tristeza virus resistance gene from Poncirus trifoliata, Genome, 40, pp. 697-704, (1997)

[8]

Deng Z., Huang S., Ling P., Chen C., Yu C., Weber C.A., Moore G.A., Gmitter F.G. Jr., Cloning and characterization of NBS-LRR class resistance gene candidate sequences in citrus, Theor Appl Genet, 101, pp. 814-822, (2000)

[9]

Deng Z., Tao Q., Chang Y.L., Huang S., Ling P., Yu C., Chen C., Gmitter F.G. Jr., Zhang H.-B., Construction of a bacterial artificial chromosome (BAC) library for citrus and identification of BAC contigs containing resistance gene candidates, Theor Appl Genet, (2001)

[10]

Dixon M.S., Jones D.A., Keddie J.S., Thomas C.M., Harrison K., Jones J.D.G., The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins, Cell, 84, pp. 451-459, (1996)

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