Immunohistochemical demonstration of apoptosis-regulated proteins, Bcl-2 and Bax, in resected non-small-cell lung cancers

被引：25

作者：

Hanaoka T. ^{[1
]}

Nakayama J. ^{[2
]}

Haniuda M. ^{[1
]}

Sato T.-A. ^{[3
]}

机构：

[1] Department of Surgery, Shinshu University, School of Medicine, Matsumoto 390-8621

[2] Department of Laboratory Medicine, Shinshu University, School of Medicine, Matsumoto

[3] Department of Otolaryngology/Head and Neck Surgery and Pathology, Columbia University, New York, NY

来源：

International Journal of Clinical Oncology | 2002年 / 7卷 / 3期

关键词：

Apoptosis-regulated proteins; Bcl-2/Bax ratio; Clinicopathology; Immunohistochemistry; Spontaneous apoptosis;

D O I：

10.1007/s101470200022

中图分类号：

学科分类号：

摘要：

Objective. Bcl-2 and Bax proteins regulate apoptosis independently, cooperatively, or both in vitro. The purpose of this study was to clarify the association between their expression with spontaneous apoptosis and various clinicopathologic features in patients with non-small-cell lung cancer (NSCLC). Methods. Bcl-2 protein, Bax protein, and spontaneous apoptosis were evaluated retrospectively in 70 resected specimens from NSCLC patients. Immunohistochemical (IHC) tests were used to assess the expression of Bcl-2 and Bax in the samples. The apoptotic index (AI) was also measured in these samples by the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) method. We then evaluated the clinicopathologic features of all 70 samples and their relationships with malignancy. Results. Bcl-2 overexpression was significantly associated with male sex and squamous cell carcinoma (SCC). Bax overexpression was not associated with any clinicopathologic features. AI was significantly associated with SCC and negative nodal metastasis. No clear associations were found among Bcl-2 expression, Bax expression, and AI. Bcl-2/Bax ratios were not associated significantly with AI. Bcl-2, Bax, Bcl-2/Bax ratio, and the grading of AI did not have prognostic values. Conclusion. Bcl-2 overexpression was significantly associated with SCC. Spontaneous apoptosis was significantly associated with SCC and with negative nodal metastasis. Apoptosis-regulated proteins, Bcl-2 and Bax, and AI lack significant associations with each other and prognostic values in patients with resected NSCLC.

引用

页码：152 / 158

页数：6

共 29 条

[1]

Korsmeyer S.J., BCL-2 gene family and the regulation of programmed cell death, Cancer Res, 59, 7 SUPPL., (1999)

[2]

Tsujimoto Y., Finger L.R., Yunis J., Et al., Cloning of the chromosome breakpoint of neoplastic B cells with the t(14

[3]

18) chromosome translocation, Science, 226, pp. 1097-1099, (1984)

[4]

Sato T., Hanada M., Bodrug S., Et al., Interactions among members of the Bcl-2 protein family analyzed with a yeast two- hybrid system, Proc Natl Acad Sci USA, 91, pp. 9238-9242, (1994)

[5]

Hanaoka T., Nakayama J., Mukai J., Et al., Association of smoking with apoptosis-regulated proteins (Bcl-2, Bax and p53) in rcsected non-small cell lung cancers, Int J Cancer, 91, pp. 267-269, (2001)

[6]

Mountain C.F., Revisions in the International System for staging lung cancer, Chest, 111, pp. 1710-1717, (1997)

[7]

Ichikawa T., Nakayama J., Sakura N., Et al., Expression of N-acetyllactosamine and β 1,4-galactosyltransferase, β 4 Gal T-I, during adenoma-carcinoma sequence in the human colorectum, J Histochem Cytochem, 47, pp. 1593-1601, (1999)

[8]

Pezzella F., Turley H., Kuzu I., Et al., Bcl-2 protein in non-small-cell lung carcinoma, N Engl J Med, 329, pp. 690-694, (1993)

[9]

Caputi M., Groeger A.M., Esposito V., Et al., Frequent high expression of bax pro-apoptotic protein in non-small cell lung cancer, Anticancer Res, 19, pp. 825-827, (1999)

[10]

Gavrieli Y., Sherman Y., Ben-Sasson S.A., Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation, J Cell Biol, 119, pp. 493-501, (1992)

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