Phenotypic analysis of bovine chondrocytes cultured in 3D collagen sponges: Effect of serum substitutes

被引：71

作者：

Yates K.E. ^{[1
,2
]}

Allemann F. ^{[1
,2
]}

Glowacki J. ^{[1
,2
]}

机构：

[1] Department of Orthopedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115

[2] Skeletal Biology Research Center, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA

来源：

Cell and Tissue Banking | 2005年 / 6卷 / 1期

基金：

美国国家卫生研究院;

关键词：

3D scaffolds; Chondrocytes; ITS; Nutridoma; Serum substitutes; Tissue engineering;

D O I：

10.1007/s10561-005-5810-0

中图分类号：

学科分类号：

摘要：

Repair of damaged cartilage usually requires replacement tissue or substitute material. Tissue engineering is a promising means to produce replacement cartilage from autologous or allogeneic cell sources. Scaffolds provide a three-dimensional (3D) structure that is essential for chondrocyte function and synthesis of cartilage-specific matrix proteins (collagen type II, aggrecan) and sulfated proteoglycans. In this study, we assessed porous, 3D collagen sponges for in vitro engineering of cartilage in both standard and serum-free culture conditions. Bovine articular chondrocytes (bACs) cultured in 3D sponges accumulated and maintained cartilage matrix over 4 weeks, as assessed by quantitative measures of matrix content, synthesis, and gene expression. Chondrogenesis by bACs cultured with Nutridoma as a serum replacement was equivalent or better than control cultures in serum. In contrast, chondrogenesis in insulin-transferrin-selenium (ITS+3) serum replacement cultures was poor, apparently due to decreased cell survival. These data indicate that porous 3D collagen sponges maintain chondrocyte viability, shape, and synthetic activity by providing an environment favorable for high-density chondrogenesis. With quantitative assays for cartilage-specific gene expression and biochemical measures of chondrogenesis in these studies, we conclude that the collagen sponges have potential as a scaffold for cartilage tissue engineering. © Springer 2005.

引用

页码：45 / 54

页数：9

共 42 条

[1]

Benya P.D., Padilla S.R., Nimni M.E., Independent regulation of collagen types by chondrocytes during the loss of differentiated function in culture, Cell, 15, pp. 1313-1321, (1978)

[2]

Benya P.D., Shaffer J.D., Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels, Cell, 30, pp. 215-224, (1982)

[3]

Boyan B.D., Hummert T.W., Dean D.D., Schwartz Z., Role of material surfaces in regulating bone and cartilage cell response, Biomaterials, 17, pp. 137-146, (1996)

[4]

Brittberg M., Lindahl A., Nilsson A., Ohlsson C., Isaksson O., Peterson L., Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation, N. Engl. J. Med., 331, pp. 889-895, (1994)

[5]

Brittberg M., Peterson L., Sjogren-Jansson E., Tallheden T., Lindahl A., Articular cartilage engineering with autologous chondrocyte transplantation. A review of recent developments, J. Bone Joint Surg. Am., 85 A, SUPPL. 3, pp. 109-115, (2003)

[6]

Chaipinyo K., Oakes B.W., van Damme M.P., Effects of growth factors on cell proliferation and matrix synthesis of low-density, primary bovine chondrocytes cultured in collagen i gels, J. Orthop. Res., 20, pp. 1070-1078, (2002)

[7]

Chaipinyo K., Oakes B.W., Van Damme M.P., The use of debrided human articular cartilage for autologous chondrocyte implantation: Maintenance of chondrocyte differentiation and proliferation in type i collagen gels, J. Orthop. Res., 22, pp. 446-455, (2004)

[8]

Farndale R.W., Sayers C.A., Barrett A.J., A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures, Connect. Tissue Res., 9, pp. 247-248, (1982)

[9]

Frondoza C., Sohrabi A., Hungerford D., Human chondrocytes proliferate and produce matrix components in microcarrier suspension culture, Biomaterials, 17, pp. 879-888, (1996)

[10]

Gerstenfeld L.C., Uporova T., Schmidt J., Strauss P.G., Shih S.D., Huang L.F., Gundberg C., Mizuno S., Glowacki J., Osteogenic potential of murine osteosarcoma cells: Comparison of bone-specific gene expression in in vitro and in vivo conditions, Lab. Invest., 74, pp. 895-906, (1996)

← 1 2 3 4 5 →