COMMUNICATION: Culture conditions producing structure entities composed of Cellulose I and II in bacterial cellulose

Culture conditions for the production of Cellulose I and/or II structures have been investigated by transmission electron microscopy using smooth colonies of Acetobacter xylinum ATCC23769. Cells prepared from smooth colonies produce the band material composed of Cellulose II in phosphate buffer (pH 7) at 4 °C. In contrast, the same cells produce the normal twisting ribbons of Cellulose I when the incubation temperature is raised to 28 °C. The band material is also produced at 4 °C in 2% buffered glucose solution and in the standard Hestrin-Schramm medium.