Transgenic sorghum plants obtained after microprojectile bombardment of immature inflorescences

被引：37

作者：

Casas A.M. ^{[1
]}

Kononowicz A.K. ^{[2
,3
]}

Haan T.G. ^{[2
]}

Zhang L. ^{[2
,4
]}

Tomes D.T. ^{[5
]}

Bressan R.A. ^{[2
]}

Hasegawa P.M. ^{[2
]}

机构：

[1] Depto. de Genet. y Prod. Vegetal, Estac. Experimental de Aula Dei, 50080 Zaragoza

[2] Ctr. Plant Environ. Stress Physiol., 1165 Horticulture Building, Purdue University, West Lafayette

[3] Dept. Plant Cytol. and Cytochemistry, University of Lodz, 90-237 Lodz

[4] South China Institute of Botany, Guanagzhou

[5] Pioneer Hi-Bred International, Inc., Johnston

来源：

In Vitro Cellular & Developmental Biology - Plant | 1997年 / 33卷 / 2期

关键词：

Biolistic; Florets; Sorghum bicolor; Transgenic plants;

D O I：

10.1007/s11627-997-0003-0

中图分类号：

学科分类号：

摘要：

Transgenic sorghum plants (Sorghum bicolor L. Moench, cv. SRN39) were obtained by microprojectile-mediated DNA delivery (Bio-Rad PDS 1000/He Biolistic Delivery System) to explants derived from immature inflorescences. Explants were precultured on medium supplemented with 2.5 mg/l (11.31 μM) 2,4-D, 0.5 mg/l (2.32 μM) kinetin, and 60 g/l sucrose for 1 to 2 wk prior to bombardment. Bialaphos selection pressure was imposed 2 wk after bombardment and maintained throughout all the culture stages leading to plant regeneration. More than 2500 explants from 1.5 to 3.0 cm inflorescences were bombarded and subjected to bialaphos selection. Out of more than 190 regenerated plants, 5 were determined to be Ignite resistant. Southern analyses confirmed the likelihood that the 5 herbicide resistant plants derived from two independent transformation events. The phosphinothricin acetyltransferase gene (bar) was inherited by and functionally expressed in T1 progeny. However, no β-glucuronidase (GUS) activity could be detected in T1 plants that contained uidA restriction fragments. Histological analyses indicated that in the absence of bialaphos morphogenesis was primarily via embryogenesis while organogenesis was more predominant in callus maintained with herbicide selection.

引用

页码：92 / 100

页数：8

共 65 条

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