Stability of Repeated Sequence Clusters in Hybrids of Maize as Revealed by FISH

被引:19
作者
James A. Birchler
Patrice S. Albert
Zhi Gao
机构
[1] University of Missouri,Division of Biological Sciences
关键词
Maize; Repetitive sequences; Knobs; Heterochromatin; rDNA; Centromeres; Genome size; FISH;
D O I
10.1007/s12042-007-9001-y
中图分类号
学科分类号
摘要
Previous studies of genome size in inbreds and hybrids had revealed that in some combinations the genome size in hybrids deviates from the midparent value of the parents. We examined whether repetitive sequence arrays such as knob heterochromatin, centromere repeats and ribosomal RNAs, as visualized cytologically in root tip chromosome spreads, would reveal any changes in copy number in such hybrids. The results indicate that no obvious changes in copy number are observed. Thus, the mechanisms by which repetitive arrays change copy number seem unrelated to the hybrid effect. Moreover, the hybrid genome effect is not manifested in gross changes in the most common repetitive sequences in the genome as determined in root tip spreads.
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[1]  
Han F(2003)Rapid genomic changes in interspecific and intergeneric hybrids and allopolyploids in Triticeae Genome 46 716-723
[2]  
Fedak G(2004)Genomic constitution and variation in five partial amphiploids of wheat- Theor Appl Genet 109 1070-1076
[3]  
Ouellet T(2005) as revealed by GISH, multicolor GISH and seed storage protein analysis Genetics 170 1239-1245
[4]  
Liu B(2004)Rapid and repeatable elimination of a parental genome-specific DNA repeat (pGc1R-1a) in newly synthesized wheat allopolyploids Plant Cell 16 571-581
[5]  
Han F(1999)Maize centromeres: organization and functional adaptation in the genetic background of oat Biotech Histochem 3 160-166
[6]  
Liu B(2006)Air drying method using nitrous oxide for chromosome counting in maize Biotech Histochem 81 71-78
[7]  
Fedak G(2004)Sensitive FISH signal detection using directly labeled probes produced by high concentration DNA polymerase nick translation in maize Proc Natl Acad Sci U S A 101 13554-13559
[8]  
Liu B(2007)Chromosome painting using repetitive DNA sequence as probes for somatic chromosome identification in maize Genetics 175 1047-1058
[9]  
Han F(1985)Single gene detection and karyotyping using small target FISH on maize somatic chromosomes Heredity 55 307-313
[10]  
Fedak G(2004)Nuclear DNA content in the genera Zea and Sorghum. Intergeneric, interspecific and intraspecific variation Genome 47 839-848