NuMA is required for proper spindle assembly and chromosome alignment in prometaphase

被引：33

作者：

Haren L. ^{[1
,2
]}

Gnadt N. ^{[1
]}

Wright M. ^{[2
]}

Merdes A. ^{[1
,2
]}

机构：

[1] Wellcome Trust Centre for Cell Biology, University of Edinburgh, King's Buildings

[2] Centre National de la Recherche Scientifique, Pierre Fabre UMR 2587, 31400 Toulouse

来源：

BMC Research Notes | / 2卷 / 1期

基金：

英国惠康基金;

关键词：

Spindle Pole; Spindle Assembly Checkpoint; Spindle Microtubule; Bipolar Spindle; Chromosome Alignment;

D O I：

10.1186/1756-0500-2-64

中图分类号：

学科分类号：

摘要：

Background. NuMA is a protein that has been previously shown to play a role in focusing microtubules at the mitotic spindle poles. However, most previous work relies on experimental methods that might cause dominant side effects on spindle formation, such as microinjection of antibodies, overexpression of mutant protein, or immunodepletion of NuMA-containing protein complexes. Findings. To circumvent these technical problems, we performed siRNA experiments in which we depleted the majority of NuMA in human cultured cells. Depleted mitotic cells show a prolonged duration of prometaphase, with spindle pole defects and with unattached, unaligned chromosomes. Conclusion. Our data confirm that NuMA is important for spindle pole formation, and for cohesion of centrosome-derived microtubules with the bulk of spindle microtubules. Our findings of NuMA-dependent defects in chromosome alignment suggest that NuMA is involved in stabilizing kinetochore fibres. © 2009 Merdes et al.

引用

共 31 条

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