Intracellular distribution and identification of the nuclear localization signals of two plant heat-stress transcription factors

被引：84

作者：

Lyck R. ^{[1
]}

Harmening U. ^{[1
]}

Höhfeld I. ^{[1
]}

Treuter E. ^{[1
]}

Scharf K.-D. ^{[1
]}

Nover L. ^{[1
]}

机构：

[1] Department of Molecular Biology, Biocenter J.W. Goethe-University

来源：

Planta | 1997年 / 202卷 / 1期

关键词：

DNA binding; Heat-stress; Lycopersicon; Nuclear localization signal; transcription factor; Transient expression assay;

D O I：

10.1007/s004250050110

中图分类号：

学科分类号：

摘要：

Similar to heat-stress transcription factors (HSFs) from non-plant sources, HSFA1 and HSFA2 from tomato (Lycopersicon esculentum Mill) contain two conserved clusters of basic amino acid residues (K/R1 and K/R2) which might serve as nuclear localization signal (NLS) motifs. Mutation of either one of them and functional testing of the corresponding proteins in a transient expression assay using tobacco (Nicotiana plumbaginifolia L.) protoplasts gave the following results. Whereas K/R1, positioned in all HSFs at the C-terminus of the DNA-binding domain, had no influence on nuclear import, the K/R1 mutants were impaired in their interaction with the DNA (band-shift assays). In contrast to this, mutants of the K/R2 motif, found 15 20 amino acid residues C-terminal of the oligomerization domain (HR-A/B region), had wild-type activity in DNA-binding but were defective in nuclear import. Thus, for the related tomato HSFA1 and HSFA2 the K/R2 cluster represents the only NLS motif, and in this function it cannot be replaced by K/R1.

引用

页码：117 / 125

页数：8

共 41 条

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