An atlas of gene expression from seed to seed through barley development

被引：113

作者：

Druka A. ^{[1
]}

Muehlbauer G. ^{[2
]}

Druka I. ^{[1
]}

Caldo R. ^{[3
,4
]}

Baumann U. ^{[5
]}

Rostoks N. ^{[1
]}

Schreiber A. ^{[5
]}

Wise R. ^{[3
,4
,6
]}

Close T. ^{[7
]}

Kleinhofs A. ^{[8
,9
]}

Graner A. ^{[10
]}

Schulman A. ^{[11
,12
]}

Langridge P. ^{[5
]}

Sato K. ^{[13
]}

Hayes P. ^{[14
]}

McNicol J. ^{[1
,15
]}

Marshall D. ^{[1
]}

Waugh R. ^{[1
]}

机构：

[1] Scottish Crop Research Institute, Dundee DD2 5DA, Invergowrie

[2] Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul

[3] Department of Plant Pathology, Iowa State University, Ames

[4] Center for Plant Responses to Environmental Stresses, Iowa State University, Ames

[5] University of Adelaide, Department of Plant Science, Glen Osmond, SA 5064, Waite Campus

[6] Corn Insects and Crop Genetics Research, USDA-ARS, Iowa State University, Ames

[7] Department of Botany and Plant Sciences, University of California, Riverside

[8] Department of Crop and Soil Sciences, Washington State University, Pullman

[9] Department of Genetics and Cell Biology, Washington State University, Pullman

[10] Institut für Pflanzengenetik und Kulturpflanzenforschung, 06466 Gatersleben

[11] MTT/BI Plant Genomics Laboratory, University of Helsinki, Helsinki

[12] MTT Agrifood Research Finland, 00014 Helsinki

[13] Research Institute for Bioresources, Okayama University

[14] Department of Crop and Soil Science, Oregon State University, Corvallis

[15] BioSS Office, Scottish Crop Research Institute, Dundee DD2 5DA, Invergowrie

来源：

Functional & Integrative Genomics | 2006年 / 6卷 / 3期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Barley; Development; Gene expression;

D O I：

10.1007/s10142-006-0025-4

中图分类号：

学科分类号：

摘要：

Assaying relative and absolute levels of gene expression in a diverse series of tissues is a central step in the process of characterizing gene function and a necessary component of almost all publications describing individual genes or gene family members. However, throughout the literature, such studies lack consistency in genotype, tissues analyzed, and growth conditions applied, and, as a result, the body of information that is currently assembled is fragmented and difficult to compare between different studies. The development of a comprehensive platform for assaying gene expression that is available to the entire research community provides a major opportunity to assess whole biological systems in a single experiment. It also integrates detailed knowledge and information on individual genes into a unified framework that provides both context and resource to explore their contributions in a broader biological system. We have established a data set that describes the expression of 21,439 barley genes in 15 tissues sampled throughout the development of the barley cv. Morex grown under highly controlled conditions. Rather than attempting to address a specific biological question, our experiment was designed to provide a reference gene expression data set for barley researchers; a gene expression atlas and a comparative data set for those investigating genes or regulatory networks in other plant species. In this paper we describe the tissues sampled and their transcriptomes, and provide summary information on genes that are either specifically expressed in certain tissues or show correlated expression patterns across all 15 tissue samples. Using specific examples and an online tutorial, we describe how the data set can be interrogated for patterns and levels of barley gene expression and how the resulting information can be used to generate and/or test specific biological hypotheses. © Springer-Verlag 2006.

引用

页码：202 / 211

页数：9

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