Building a bilayer model of the neuromuscular synapse

被引：5

作者：

Woodbury D.J. ^{[1
]}

机构：

[1] Department of Physiology, Wayne Stt. Univ. School of Medicine, Detroit, MI

来源：

Cell Biochemistry and Biophysics | 1999年 / 30卷 / 3期

基金：

美国国家卫生研究院;

关键词：

ACh; AChR; synaptic vesicle; Ca; -channel; vesicle fusion; reconstitution;

D O I：

10.1007/BF02738117

中图分类号：

学科分类号：

摘要：

Progress over the past 10 years has made it possible to construct a simple model of neurotransmitter release. Currently, some models use artificially formed vesicles to represent synaptic vesicles and a planar lipid bilayer as a presynaptic membrane. Fusion of vesicles with the bilayer is via channel proteins in the vesicle membrane and an osmotic gradient. In this paper, a framework is presented for the successful construction of a more complete model of synaptic transmission. This model includes real synaptic vesicles that fuse with a planar bilayer. The bilayer contains acetylcholine receptor (AChR) channels which function as autoreceptors in the membrane. Vesicle fusion is initiated following a Ca2+ flux through voltage-gated Ca2+ channels. Key steps in the plan are validated by mathematical modeling. Specifically, the probability that a reconstituted AChR channel opens following the release of ACh from a fusing vesicle, is calculated as a function of time, quantal content, and number of reconstituted AChRs. Experimentally obtainable parameters for construction of a working synapse are given. The inevitable construction of a full working model will mean that the minimal structures necessary for synaptic transmission are identified.

引用

页码：303 / 329

页数：26

共 74 条

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[2]

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[3]

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[9]

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[10]

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