A rapid and high yielding DNA miniprep for cotton (Gossypium spp.)

被引:34
作者
Haiwen Li
Jinhua Luo
John K. Hemphill
Jau-Tay Wang
Jean H. Gould
机构
[1] Texas A&M University,Department of Forest Science
[2] AgriVitis,undefined
[3] Inc.,undefined
关键词
DNA extraction; minipreparation;
D O I
10.1007/BF02772162
中图分类号
学科分类号
摘要
A rapid DNA minipreparation method was developed for cotton and yields 500–600 μg DNA from 1.0 g fresh leaf tissue. Cotton DNA extracted using this method is completely digested with restriction enzymes, supports PCR and Southern DNA analyses and was used successfully in these applications.
引用
收藏
页码:183 / 183
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