Pilot scale production of pory(3-hydroxybutyrate-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli

被引：24

作者：

Choi J.-I. ^{[1
,2
]}

Lee S.Y. ^{[1
,2
,3
]}

Shin K. ^{[2
]}

Lee W.G. ^{[2
]}

Park S.J. ^{[1
,2
]}

Chang H.N. ^{[2
]}

Chang Y.K. ^{[2
]}

机构：

[1] Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701

[2] Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Yuseong-gu, Daejeon 305-701

[3] Center for Ultramicrochemical Process Systems, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701

来源：

Biotechnology and Bioprocess Engineering | 2002年 / 7卷 / 6期

关键词：

Oxygen transfer rate; P(3HB/V); Pilot-scale fermentor; Recombinant E. coli;

D O I：

10.1007/BF02933524

中图分类号：

学科分类号：

摘要：

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB/V)], by fed-batch culture of recombinant Escherichia coli harboring a plasmid containing the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only. In a 30 L fermentor having a KLa value of 0.11 s-1, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively, giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having a KLa of 0.03 s-1, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively, giving a productivity of 1.06 g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinant E. coli in a large-scale fermentor having low KLa value.

引用

页码：371 / 374

页数：3

共 14 条

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