A comparison of two-dimensional electrophoresis data with phenotypical traits in Arabidopsis leads to the identification of a mutant (cri1) that accumulates cytokinins

被引：3

作者：

Véronique Santoni

Marianne Delarue

Michel Caboche

Catherine Bellini

机构：

[1] Biochimie et Physiologie Végétales,

[2] INRA/ENSA-M/CNRS URA 573,undefined

[3] F-34060 Montpellier Cedex 1,undefined

[4] France,undefined

[5] INRA Laboratoire de Biologie Cellulaire,undefined

[6] route de Saint-Cyr,undefined

[7] F-78026 Versailles Cedex,undefined

[8] France,undefined

来源：

Planta | 1997年 / 202卷

关键词：

Key words:Arabidopsis (mutants) ; Biochemical classi-fication of mutants ; Mutant (Arabidopsis);

D O I：

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中图分类号：

学科分类号：

摘要：

Total proteins extracted from developmental mutants of Arabidopsis thaliana (L.) Heyhn. and from wild-type plants cultivated in the presence of various hormones were analyzed by two-dimensional (2-D) gel electrophoresis. Computer analysis of 2-D gels followed by a statistical treatment of data allowed us to build a phenogram that describes the biochemical distances between the different genotypes. Analysis of the 2-D electrophoresis data allowed us to discriminate mutants in agreement with phenotypical and physiological traits. This biochemical analysis helped us to develop a working hypothesis which led us to show that one developmental mutant (cri1 ) overaccumulates cytokinins.

引用

页码：62 / 69

页数：7