Yeast two-hybrid: State of the art

被引：67

作者：

Van Criekinge W. ^{[1
]}

Beyaert R. ^{[2
]}

机构：

[1] Devgen N.V. Technologiepark 9

[2] Department of Molecular Biology, Flanders Interuniversity Institute for Biotechnology, University of Ghent

来源：

Biological Procedures Online | 1999年 / 2卷 / 1期

关键词：

Yeast Cell; Reporter Gene; Biological Procedure; Library Plasmid; Upstream Activate Sequence;

D O I：

10.1251/bpo16

中图分类号：

学科分类号：

摘要：

Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a "differential" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to "real" antibodies. With the technology available, the only limitation is imagination. ©1999 Biological Procedures Online. All rights reserved.

引用

页码：1 / 38

页数：37

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