The spatial localization of T-DNA insertions in petunia interphase nuclei: Consequences for chromosome organization and transgene insertion sites

被引：11

作者：

Ten Hoopen R. ^{[1
,2
]}

Montijn B.M. ^{[1
]}

Veuskens J.T.M. ^{[1
,3
]}

Oud O.J.L. ^{[1
]}

Nanninga N. ^{[1
]}

机构：

[1] Inst. for Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, 1098 SM Amsterdam

[2] Inst. for Plant Genet./Crop Plant R., 64466 Gatersleben

[3] Kreatech Diagnostics, 1100 AT Amsterdam

来源：

Chromosome Research | 1999年 / 7卷 / 8期

关键词：

Confocal microscopy; Fluorescence in-situ hybridization; Petunia hybrida; Transgenes;

D O I：

10.1023/A:1009232002104

中图分类号：

学科分类号：

摘要：

In an earlier fluorescent in-situ hybridization (FISH) study on petunia, we found a considerable discrepancy between the genetic map and the physical map with respect to T-DNA insertions on metaphase chromosomes. For some transgenes we found a preference to integrate near the telomeres. Here, we studied the spatial position of transgenes in interphase nuclei by FISH and 3D-confocal microscopy to elucidate a possible structural preference for the nuclear localization of transgenes. Three transgenes located near telomeres on three different metaphase chromosomes showed a much more internal distribution in interphase root meristem than the telomeres, whereas a proximal transgene appeared to be distributed in a random fashion. The results point to local differences in chromatin compacting along a chromosome. These differences might explain a preference for T-DNA insertion in distal regions of the chromosome.

引用

页码：611 / 623

页数：12

共 15 条

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