Assessment of the uniformity of wheat and tomato varieties at DNA microsatellite loci

被引:1
作者
R.J. Cooke
G.M.M. Bredemeijer
M.W. Ganal
R. Peeters
P. Isaac
S. Rendell
J. Jackson
M.S. Röder
V. Korzun
K. Wendehake
T. Areshchenkova
M. Dijcks
D. Laborie
L. Bertrand
B. Vosman
机构
[1] NIAB,
[2] Plant Research International,undefined
[3] Institute for Plant Genetics and Crop Plant Research (IPK),undefined
[4] TraitGenetics GmbH,undefined
[5] Nunhems Zaden B.V.,undefined
[6] Agrogene SA,undefined
[7] CTRC-IDD,undefined
来源
Euphytica | 2003年 / 132卷
关键词
microsatellites; SSR; tomato; uniformity; variety testing; wheat;
D O I
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中图分类号
学科分类号
摘要
By analysing a number (20–38) of individuals from selected varieties of wheat and tomato, we have been able to assess intra-varietal uniformity at certain micro satellite (simple sequence repeat, SSR) loci. In total, 45 varieties of wheat were analysed at between 7–9 different SSR loci, and 10 varieties of tomato were analysed at six loci. The results showed that there was variation both between varieties and between microsatellites in the degree of non-uniformity observed, and it was possible to identify a number of different probable sources of non-uniformity. Twenty-four of the wheat varieties and nine of the tomato varieties were sufficiently uniform to meet the standards currently applied for distinctness, uniformity and stability (DUS) testing using phenotypic characteristics. The implications for the potential future use of SSRs in DUS testing are discussed.
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页码:331 / 341
页数:10
相关论文
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